Navegando por Palavras-chave "Transfusão De Eritrócitos"

Agora exibindo 1 - 2 de 2
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Influência de características do armazenamento em parâmetros hematológicos e bioquímicos de concentrados de glóbulos vermelhos
    (Universidade Federal de São Paulo (UNIFESP), 2019-03-28) Jardini, Elen Francini [UNIFESP]; Pedreira, Mavilde Da Luz Goncalves [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Long storage periods can compromise red blood cells integrity. Thus, a classification of package red blood cells according to storage time has been investigated and used in the transfusion practices. The complexity of factors involving the transfusion process and concerns of possible deleterious effects in the transfused patient grounded this investigation regarding the quality of the blood component to be transfused. Objectives: To describe hematological and biochemical parameters of red blood cells and to verify the influence of storage and pre infusion temperature, bag concentrates brand and storage time on hematological and biochemical parameters. Materials and Methods: Descriptive research developed with secondary data of experimental studies accomplished in the Laboratory of Nursing Experiments at the Federal University of São Paulo, between 2015 and 2016. The following parameters were studied in a total of 82 red blood cells: total hemoglobin (g/dL), free hemoglobin (g/dL), hematocrit (%), degree of hemolysis (%), potassium (mmol/L), lactic dehydrogenase (U/L), haptoglobin (g/L), pH and osmolality (mOsm/Kg). For the statistical analysis arithmetic mean, standard deviation, median and interquartile range were used, and the tests of variance, Skewness and Kurtosis, D'Agostino & Pearson, Kolmogorov-Smirnov and Shapiro-Wilk were applied, t Student, Mann-Whitney, One-Way ANOVA (p <0,05). Results: Among the 82 units, 79 (96.34 %) had blood typing A+, 2 (2.44 %) O+ and 1(1.22 %) typing AB+. Regarding the bags brands, 68.29 % were Grifols®, 28.05 % JP Farma® and 3.66 % Fresenius®. There was correlation between higher initial temperature and alterations in levels of lactic dehydrogenase (p=0.040) and osmolality (p=0.020); higher preinfusion temperature and higher levels of free hemoglobin (p=0.003), hematocrit (p=0.002) and osmolality (p=0.020). The red blood cells in the Grifols® bags’ brand showed a statistically significant difference in the potassium level (p = 0.020). In relation to the storage time, longer periods lead to higher levels of free hb levels (p <0.0001), degree of hemolysis (p<0.0001), potassium (p=0.010), lactic dehydrogenase (p=0.0001) and decreased pH (p <0.0001) and haptoglobin levels (p=0.004). Conclusion: The majority of the studied red blood cells presented central tendence measureaments of hematological and biochemical parameters according to national and international standards; red blood cells submitted to higher temperatures and storage for longer periods presented more variations.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Marcadores de hemólise de concentrado de hemácias após infusão in vitro por cateter intravenoso periférico
    (Universidade Federal de São Paulo (UNIFESP), 2019-12-18) Miyasaki, Vanessa Yukie Kita [UNIFESP]; Avelar, Ariane Ferreira Machado [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: The infusion of red blood cells (RBC) through peripheral intravenous catheters is widely performed in clinical practice in hospitalized individuals. The literature indicates that storage time as well as infusion devices used in this process may interfere with the levels of hemolysis markers’ level. Objective: To identify and compare the influence of the storage time of packed red blood cells, the infusion rate and the caliber of peripheral intravenous catheter on the levels of hemolysis markers after in vitro infusion of the red blood cells. Material and Method: Experimental study conducted under controlled environmental conditions, which was used the excess of the RBC pack preserved in CPDA-1 in blood type A+ stored at Collection Association COLSAN, the samples were randomly administered in quintuplicate to each peripheral intravenous cateters 14,18 e 20G in manually controlled dripping at 16 and 33 drops/min. The RBC pack had storage time shorter and longer than 14 days. The researchers colleted samples directly from the bag (V1), after this, the infusion set and the catheter was filled through free-flow. The hemolytic markers analyzed were: degree of hemolysis (%), hematocrit-Ht (%), total hemoglobin-HbT (g/dl), free hemoglobin-HbL (g/dl), potassium-K (mmol/l) and lactate dehydrogenase-LDH (U/L). For data analysis, statistical tests were used according to the nature of the variables (ANOVA, Kruskall-Wallis and Generalized Estimating Equation), considering significant p<0,05. Results: We perfomed 60 experiments with analysis of 180 aliquots obtained from 11 units of RBC concentrate. The RBC packs stored for less than 14 days tended to increase in HbT, HbL and K values regardless of catheter’s size (p>0,05); whereas in older than 14 days RBC bags, the same hemolytic markers were identified, with a statistically significance difference (p=0,031) only in the Ht levels. The lower infusion rate (16 drops/min) significantly influenced the decrease in the K level. The infusion rates did not show significant influence on hemolysis markers. Regarding the catheters caliber, considering the difference between the values of V1 and V2, there was no influence of the three catheters’ size on HbT (<0,001), HbL (p<0,001) and on the degree of hemolysis (p=0,001). In the 18G caliber, greater variations in HbL and degree of hemolysis were identified. Conclusion: When analyzing the influence of each CIVP caliber, it was observed discreet changes in hemolysis’ markers levels, however, the 18 G caliber significantly influenced HbL levels and degree of hemolysis, and in the 14 G influenced HbT levels after controlled drop infusion. Storage time of CH packs greater then 14 days significantly influenced Ht levels after the blood passed through the infusion line in free flow. In the comparison between the infusion rates, a significant influence on the K at lower velocity was identified with significant decrease. The levels of hemolysis’ markers found do not contraindicate the CH administration under the experimental consitions evaluated in this study.