Navegando por Palavras-chave "Tecido Adiposo Marrom"

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    Avaliação preliminar da produção de melatonina no tecido adiposo marrom
    (Universidade Federal de São Paulo (UNIFESP), 2019-12-18) Olescuck, Ingrid Fernandes [UNIFESP]; Amaral, Fernanda Gaspar Do [UNIFESP]; http://lattes.cnpq.br/6398402888086154; http://lattes.cnpq.br/1821363446203117; Universidade Federal de São Paulo (UNIFESP)
    Objective: Verify the existence of local melatonin production in brown adipose tissue. Methods: We realized three experimental models. In experiment I the animals were euthanized every three hours during 24 hours, with no challenge to the interscapular brown adipose tissue (BAT IE). In experiment II the animals were exposed to hipercaloric food over 24h or to cold temperature (13°C) during 3 hours before euthanasia at ZT10. In experiment III the animals were euthanized at ZT18 and there was no additional challenge to the tissue. In all three experiments the animals were assigned to three groups: control, pinealectomized (PINX) and pinealectomized with oral melatonin replacement (PINX/MEL). We collected BAT IE from all animals for the analyses. Results: mRNA from TPH1 enzyme was accessed by PCR reaction in control, PINX and PINX/MEL groups. However it was not possible to standardize the PCR and the Western Blot reactions for AANAT and HIOMT enzymes. In contrast, immunohistochemistry showed the presence of the proteins from both these enzymes (AANAT and HIOMT) in samples obtained by experiment III. Also, we were able to verify AANAT activity in BAT IE from samples obtained for tests, but we were not able to perform this procedure in our experimental samples due to technical limitations. Discussion: Despite all the technical challenges, the present data strongly suggests the presence of local melatonin production in BAT IE. However, more studies are needed to fully prove that.
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    Suplementação Com A Polpa Do Fruto De Juçara (Euterpe Edulis Mart) Durante A Gestação E Lactação Sobre A Inflamação E Expressão Da Proteína Desacopladora 1 (Ucp-1) No Tecido Adiposo Marrom E Função Mutagênica No Fígado Da Prole Aos 21 Dias
    (Universidade Federal de São Paulo (UNIFESP), 2017-03-17) Argentato, Perla Pizzi [UNIFESP]; Pisani, Luciana Pellegrini [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: The fruit of the jussara palm (Euterpe edulis Mart.) is a species native to Brazil. Studies investigating jussara consumption are least explored at literature, especially under fetal metabolic programming. Objective: To investigate the effect of maternal supplementation with jussara fruit pulp in rats treated with hydrogenated vegetable fat (HVF) during gestation and lactation on the expression of decoupling protein 1 (UCP-1), inflammatory markers in brown adipose tissue (BAT) and mutagenic activity in the liver of 21-day-old of offspring. Methods: The groups were composed of male Wistar rats: Group C - offspring of mothers who received control diet containing soybean oil. Group CJ - offspring of mothers who received C diet with 0.5% supplementation the lyophilized jussara pulp (J). Group T - offspring of mothers who received a diet containing HVF, rich in trans fatty acids (TFAs) and TJ Group - offspring of mothers who received HVF containing diet plus 0.5% of J. Growth and weight gain were evaluated, serum concentrations of HDL-cholesterol, total cholesterol (TC), triglycerides (TG) and glucose, protein contents, the carcass lipid, e TNF-α, IL-10 and IL-6 cytokines, the protein expression of UCP-1, NFκBp50 and RITNF-α in the BAT and the histopathological changes, cytogenetics and immunoreactivity of TNF-α and COX-2 in the liver. To verify the effect the treatment and comparison between the groups, the two-way ANOVA followed by Bonferroni's post hoc test was used. The results were expressed as mean ± standard error of the mean(SEM) and the significance level was p≤0.05. Results: We found that TFAs promoted birth weight gain in first and second week of lactation (TJ> CJ), reduced carcass protein (T C), the concentrations of CT (T> C; TJ> CJ) and TG (T> C) also increased TNF-α (T> C; TJ> CJ), RITNF-α (T> CJ) and UCP-1 (TJ> CJ) in BAT, altered histopathology (T> C), increased the frequency of micronuclei (T> C; T> TJ), immunoexpression to TNF and COX-2 (T> C) in the liver of offspring. In contrast supplementation with jussara reduced the weight gain in the third week of lactation and the total weight gain (CJ T), reduced carcass lipid (CJ T), decreased glycemic (TJ C) and UCP-1 (TJ> T) in BAT, decreased the number of micronucle the hepatocytes (TJ