Navegando por Palavras-chave "TNF-α"

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    Efeitos da suplementação com melatonina sobre a expressão de adipocinas por tecidos adiposos branco em camundongos obesos
    (Universidade Federal de São Paulo, 2017-07-03) Paixão, Regislane Ino da [UNIFESP]; Vale, Maria Isabel Cardoso Alonso [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Several metabolic disorders such as obesity, type 2 diabetes mellitus (DM2) and metabolic syndrome have a direct association with white adipose tissue (WAT) dysfunction and, consequently, adipokines secretion, especially those with a proinflammatory action. The incidence of obese people worldwide is increasing every year and this is an indicator that the therapeutic strategies used are not yet fully effective. Studies have shown that melatonin is a hormone responsible for ensuring an adequate energy balance, mainly through the regulation of energy flow, regulating this process, probably by modulating the WAT function. In this work we evaluated the effects of melatonin supplementation on the production of adipokines in visceral and subcutaneous fat (peri-epididimal -PE and inguinal- ING, respectively) of animals submitted to obesity by a high fat diet (HFD). Male C57Bl / 6j mice of 8 weeks were used, which were separated into 3 groups: CO, animals fed with control diet (9% of lipids); Obese, animals fed with HFD (59% of lipids) and obese + melatonin, animals fed with HFD and supplemented with melatonin (1mg/kg, drinking water for ten weeks during the dark phase). The animals were evaluated for food intake, body mass gain, and glucose tolerance test (GTT) and insulin (ITT) and plasma lipid profile. In the end of protocol, inginal and subcutaneous adipose tissue was removed to evaluate, adipokines and gene expression (adiponectin, IL-6, leptin, resistin and TNF-α). The body mass gain data showed that melatonin had a preventive effect on the development of obesity. In addition, supplementation with melatonin slowed down the effects on food efficiency and energy efficiency triggered by HFD. Consequently, the ING and PE fat depots of the animals that ingested HFD and melatonin showed significant reductions when compared to animals that were not supplemented with this hormone. The analyses of gene expression in the subcutaneous and visceral depots showed that obesity caused an increase in the content of IL-6, leptin and TNF-α in the visceral depot. However, this effect was prevented in the group of obese animals that received melatonin supplementation. Finally, melatonin attenuated the elevation of serum triglyceride levels and the LDL cholesterol fraction caused by DHL. Thus, the results obtained in this study suggest that supplementation with melatonin may decrease the weight gain and the proinflammatory effects triggered by DHL, as well as the development of atherosclerosis. Key words: obesity; pineal gland; adiponectin; leptin; TNF-α; IL-6.
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    TNF-α and IL-6 immunohistochemistry in rat renal tissue experimentaly infected with Leptospira interrogans serovar Canicola
    (Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, 2008-01-01) Marinho, Márcia; Monteiro, Cristina Maria Rodrigues; Peiró, Jr; Machado, Gf; Oliveira-Júnior, Itamar Souza [UNIFESP]; Universidade Estadual Paulista (UNESP); Universidade Federal de São Paulo (UNIFESP)
    Leptospirosis is a public health problem worldwide and its etiology remains unclear. Its pathogenesis involves a complex interaction between host and infecting microorganism. The inflammatory reaction that controls the infection process also underscores many pathophysiological events occurring in leptospirosis. We investigated the presence of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in renal tissues by immunohistochemical and histopathological examination in animals experimentally inoculated with Leptospira serovar Canicola. All the tests were carried out 2, 7, 14, 21 or 28 days after inoculation. Although TNF-α and IL-6 had been detected in tissues throughout the observation period, these cytokines appeared more intensely during the initial phase of infection. Therefore, both TNF-α and IL-6 were associated with the immunopathogenesis of leptospirosis. This profile suggests a high immunocellular response throughout the early infection stages followed by subsequent humoral response.