Navegando por Palavras-chave "Recombinant allergens"

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    In Search of a Vaccine for Mouse Allergy: Significant Reduction of Mus m 1 Allergenicity by Structure-Guided Single-Point Mutations
    (Karger, 2012-01-01) Ferrari, Elena; Breda, Daniela; Longhi, Renato; Vangelista, Luca; Nakaie, Clovis Ryuichi [UNIFESP]; Elviri, Lisa; Casali, Emanuela; Pertinhez, Thelma A.; Spisni, Alberto; Burastero, Samuele E.; Ist Sci San Raffaele; Univ Parma; CNR; Universidade Federal de São Paulo (UNIFESP)
    Background: Mouse urinary proteins are relevant allergens from mice urine. We used the recombinant protein Mus m 1 as an allergen model to identify if, by altering Mus m 1 architecture via single-point mutations, we could effectively modify its allergenicity. Methods: Based on structural considerations, we synthesized two single-point mutants, Mus m 1-Y120A and Mus m 1-Y120F, which were expected to harbor large structural alterations. Circular dichroism and fluorescence analysis showed significant conformational rearrangements of the aromatic side chains in the internal cavity of Mus m 1-Y120A when compared to Mus m 1-Y120F and Mus m 1. Evaluation of the allergenic potential of the recombinant molecules was performed in vitro with both immunochemical approaches and assays based on the measurement of basophil degranulation. Moreover, to assess the integrity of the T cell epitopes and as an in vitro measure of immunogenicity, we tested the reactivity of T lymphocytes from subjects allergic to mouse urine against proteins and synthetic peptides encompassing the immunodominant linear epitope containing the mutation. Results: We found that the selected point mutation was able to modulate the protein allergenicity, and to severely impair the recognition of Mus m 1 by IgE, while T cell reactivity was fully maintained. Conclusions: in silico predicted, minimum selected structural modifications allowed to design one protein with reduced allergenicity and preserved immunogenicity. Structurally guided mutations can direct the design of proteins with reduced allergenicity which can be used as vaccines for a safer and more effective immunotherapy of allergic disorders. Copyright (C) 2011 S. Karger AG, Basel
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    Profile of Latex Sensitization and Allergies in Children and Adolescents With Myelomeningocele in Sao Paulo, Brazil
    (Esmon Publicidad S A, 2013-01-01) Bueno de Sa, A. [UNIFESP]; Faria Camilo Araujo, R. [UNIFESP]; Cavalheiro, S. [UNIFESP]; Carvalho Mallozi, M. [UNIFESP]; Sole, D. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Background: Latex allergy is an important cause of occupational allergy. In many countries the prevalence of latex allergy is still high and the profile of latex sensitization is unknown.Objectives: To evaluate the frequency of sensitization and allergy to latex in children and adolescents with myelomeningocele and to identify associated risk factors.Methods: The study included 55 children and adolescents with myelomeningocele followed at a specialized center. In addition to a standard questionnaire and skin prick tests (SPTs) to aeroallergens and total latex, the patients underwent determination of total and specific serum IgE to latex and recombinant allergens.Results: We observed a prevalence of 25% for latex sensitization and of 20% for latex allergy. Twenty-four patients (43.6%) were atopic and the average age for the first reaction to latex was 44.5 months. Cutaneous reactions were the most frequently reported reactions (72.7%). Specific immunoglobulin (Ig) E to rHey b 1, rHev b 3, rHev b 5, rHey b 6.1, and rHev b 6.2 was detected in over 50% of patients allergic,to latex. Multivariate analysis showed current asthma, atopy, and having undergone 4 or more operations to be risk factors for latex sensitization.Conclusions: Our study documented a high prevalence of sensitization and allergy to latex in patients with myelomeningocele. Specific IgE to rHey b 1, rHey b 3, rHev b 5, rHey b 6.1, and rHey b 6.2 was detected in over 50% of children and adolescents with myelomeningocele who are allergic to latex. A history of current asthma, atopy, and having undergone 4 or more operations were independent risk factors for latex allergy.