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    Caracterização das proteases secretadas por Bacillus sp e encontradas no chorume obtidos da compostagem da fundação do parque zológico de São Paulo
    (Universidade Federal de São Paulo (UNIFESP), 2017-01-31) Santos, Saara Maria Batista Dos [UNIFESP]; Juliano Neto, Luiz [UNIFESP]; http://lattes.cnpq.br/6593457386037361; http://lattes.cnpq.br/0843281237524201; Universidade Federal de São Paulo (UNIFESP)
    Composting is defined as an active degradation process carried out by microrganisms in optimal conditions. The process can be divided into two distinct phases: the first is the thermophilic phase, which reaches approximately 45-65° C and the second stage involves maturation, resulting in the transformation in the humus material. From the prospect of composting Park Zoo Foundation of São Paulo in July 2013, it was possible to isolate and identify various microorganisms, where the genus Bacillus ssp accounted for 67% of the isolates. Of these, eight were separated Bacillus spp, and by biochemical tests, we observed enzymatic activity in the crude extract front substrates for proteases. The species were collected from a single compost: 3 species collected during the initial stage of degradation, 2 at the end of the process and other '3 between the two stages. The aim of this study was to investigate the biochemical profile of secreted proteases of the eight Bacillus isolates composting FPZSP. Achievements: enzyme activity tests were made with the FRET peptide substrate Abz-KLlSSKQ-EDDnp, secreted by the Bacillus culture in the presence of PMSF and EDTA inhibitors, which verified the presence of metal and serine proteases. Furthermore, the effect of the NaCI salt in the proteolytic activity of the secreted was also tested, where tolerance in concentrations of up to 5M was seen in some strains. The manure that runs through the composters was also separate in order to verify if there were any biochemical profile that percolate. Results: Enzyme activity was verified with the FRET-peptide substrates, which may be the same Bacillus enzymes secreted during the composting process.