Navegando por Palavras-chave "Nephrectomy 5/6 And Chronic Kidney Disease."

Agora exibindo 1 - 1 de 1
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Efeitos da aplicação de vesículas extracelulares em ratos com doença renal crônica por Nx5/6 submetidos ao exercício físico
    (Universidade Federal de São Paulo (UNIFESP), 2019-01-31) Luiz, Rafael Da Silva [UNIFESP]; Borges, Fernanda Teixeira [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The repairing effect of mesenchymal stromal cells (MSCs), especially their extracellular vesicles (VEs), has received much attention. Additionally, knowledge about physical exercise has been growing, but the effect of its association with potential repair therapies is still little explored. The objective of this study was to evaluate the effect of the association between exercise and VEs on renal function in rats submitted to Nx5/6. Wistar rats were divided into control groups (C), Exercise (E), Sedentary + Nx5/6 (NS), Exercise + Nx5/6 (NS), NS + VE (NSV) and NE + EV (ENV). The training was 60min/day of swimming, 5 days per week for 8 weeks and the VEs (100μg) were injected into the caudal vein every 10 days for 50 days, both after surgery. Our results showed that Nx5/6 increased mean arterial pressure, glomerulosclerosis and proteinuria, leading to a decrease in renal function (decreased creatinine clearance and increased plasma urea), weight gain and cardio-respiratory capacity, causing an increase in mortality. Physical exercise increased cardiorespiratory capacity, improved renal function, but not proteinuria, showed antioxidant action by inducing mitochondrial dynamics (increased fusion and decreased mitochondrial fission), which reduced the mortality of nephrectomized animals. Extracellular vesicles extracted from MSCs also increased cardio-respiratory capacity, improved kidney function and decreased mortality in nephrectomized animals. The association was beneficial, since it potentiated the effects induced by the VEs. Our results suggest that physical exercise and administration of extracellular vesicles extracted from MSCs exert protective and/or repairing effects on the kidney and decrease the mortality of nephrectomized animals, probably by distinct mechanisms that may be potentiated by each other.