Navegando por Palavras-chave "In Situ Hybridization"

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    Papilomavírus humano transcricionalmente ativo no câncer de laringe glótico
    (Universidade Federal de São Paulo (UNIFESP), 2019-05-30) Cunha, Gustavo Barreto Da [UNIFESP]; Biase, Noemi Grigoletto De [UNIFESP]; Haddad, Leonardo; Artigiani Neto, Ricardo; http://lattes.cnpq.br/3651841133236053; http://lattes.cnpq.br/7014919949145135; http://lattes.cnpq.br/9388533427918460; http://lattes.cnpq.br/9388533427918460; Universidade Federal de São Paulo (UNIFESP)
    Objective: To evaluate the association of human papillomavirus in its active form and invasive glottic laryngeal cancer. Method: We conducted a case-control study to evaluate the association of transcriptionally active human papillomavirus in patients diagnosed with glottic laryngeal squamous cell carcinoma and vocal cord polyps as cancer-free controls. p16INK4a Immunohistochemistry and DNA in situ hybridization were used to identify positive cases for the virus. Results: In total, 132 subjects were included in the study, 66 patients with laryngeal glottic squamous cell carcinoma and 66 participants in the control group. Among the individuals in the study group, only 8 were women and had a mean age of 62.3 years. Smoking and smoking load were positively associated with laryngeal cancer. p16INK4a immunohistochemistry was positive in 5 of the 66 (7.6%) patients with squamous cell carcinoma, whereas no case among controls was positive. On the other hand, all of the 5 cases presented negative in situ hybridization for human papillomavirus DNA and were therefore classified as negative human papillomavirus status. p16INK4a immunohistochemistry was not significantly associated with glottic laryngeal squamous cell carcinoma or human papillomavirus status, nor with any specific group, although there was a tendency to associate with the laryngeal cancer group and patients who never smoked. Conclusion: Transcriptionally active papillomavirus did not associate with glottic laryngeal cancer.