Navegando por Palavras-chave "Endothelial Progenitor Cells"
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- ItemSomente MetadadadosAssociação entre micropartículas endoteliais de origem apoptótica, funcionalidade de células progenitoras endoteliais e grau de aterosclerose avaliado pelo Índice de Friesinger(Universidade Federal de São Paulo (UNIFESP), 2020-10-29) Mota, Isabela Cardoso Pimentel [UNIFESP]; Franco, Maria Do Carmo Pinho [UNIFESP]; Universidade Federal de São PauloIntroduction: Apoptotic endothelial cell-derived microparticles (A-EMPs) and endothelial progenitor cells (EPCs) have shown relevance in cardiovascular pathogenesis related to endothelial dysfunction and atherosclerosis. A-EMPs are released from cell membranes during cell apoptosis and EPCs are directly involved in vascular regeneration. Objectives: To evaluate A-EMPs circulating number and EPCs function in individuals submitted to elective coronary angiography and correlate with aterosclerosis burden. Methods: 57 adult and elderly patients were submitted to elective coronary angiography without previous invasive coronary treatment. Flow cytometry was used to identify CD9+ / annexin V+,CD 144+, CD 31+ A-MPEs. CPEs’ function was defined by the number of Colony Forming Units (CFU-Hill). Atherosclerotic burden was assessed by Friesinger Index. Results: There was a positive association between A-EMPs circulating levels and atherosclerotic burden (rho = 0.817, P <0.001), while for EPCs’ function, inversely proportional result was recorded (rho = - 0.649, P <0.001). Increased A-EMPs number was significantly associated with moderate/ severe coronary atherosclerosis (OR: 1.7; 95% CI: 1.23 - 2.28; P = 0.001) as well the increased UFC-Hill EPCs (OR: 0.92; 95% CI: 0.89 - 0.97; p = 0.001). Conclusion: Circulating A-EMPs were independent atherosclerotic lesion severity predictor, while EPCs function was protective factor against moderate/severe coronary atherosclerosis in high cardiovascular risk patients. These results indicate the potential role of MPE-APs and CPEs as new markers of atherosclerotic disease,
- ItemSomente MetadadadosChallenges facing the use of endothelial progenitor cells in stem cell therapies(Assoc Arquivos Neuro- Psiquiatria, 2016) Franca, Carolina N. [UNIFESP]; Amaral, Jonatas B. [UNIFESP]; Tuleta, Izabela D.; Siviero, Fabio; Ferreira, Carlos E. S. [UNIFESP]; Izar, Maria C. O. [UNIFESP]; Fonseca, Francisco A. H. [UNIFESP]The paper summarizes the difficulties to study the rare population of endothelial progenitor cells in clinical trials, based on the experience of our group in many publications in this area.
- ItemSomente MetadadadosAs Implicações Da Restrição De Crescimento In Utero Sobre A Reatividade Vascular E As Células Progenitoras Endoteliais Em Ratos Wistar: O Papel Do Treinamento Aeróbio(Universidade Federal de São Paulo (UNIFESP), 2017-06-30) Oliveira, Vanessa Aparecida De [UNIFESP]; Franco, Maria Do Carmo Pinho [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Intrauterine growth restriction (IUGR) can induce deleterious changes in the modulatory ability of the vascular endothelium, contributing to an increased risk of developing cardiovascular diseases in the long term. However, the mechanisms by which IUGR promotes these changes are not elucidated. Considering the functional properties of endothelial progenitor cells (EPCs) in promoting endothelial repair our first hypothesis is that negative adaptations in EPCs may be involved in endothelial dysfunction induced by IUGR. Therefore, our goals were to investigate the effects of IUGR on vascular reactivity in aortic artery, number, functional properties and senescence process of in vitro CPEs in adult male offspring. For this, pregnant Wistar rats received ad libitum diet or 50% of diet ad libitum during the gestational period. Our results showed that offspring submitted to IUGR had a significant reduction of acetylcholine (ACh) -mediated vasodilation in the thoracic aorta rings, which may be, in part, to the lower concentration of nitric oxide (NO) observed in this vascular bed. The number of circulating and bone marrow EPCs was similar between the experimental groups. However, in vitro functional capacity of circulating CPEs and bone marrow was reduced in the restricted group compared to controls. Additionally, we observed that the restricted animals presented a significant increase in the number of senescent EPCs in the bone marrow vs control group. Therefore, this set of data demonstrates that IUGR can induce endothelial dysfunction in male offspring, which may be due in part to the reduction in NO concentration as well as the lower EPCs repair capacity. Based on these findings, and considering that aerobic training (AT) can induce beneficial effects on vascular function and on the functional capacity of CPEs, our second hypothesis is that the TA could reverse the deleterious adaptations in vascular function and in EPCs in IUGR rats. Therefore, we evaluated the effects of AT on vascular reactivity in aortic artery, number, functional properties and senescence process of in vitro EPCs in adult male offspring submitted to IUGR. Adult male offspring were divided into groups: sedentary control, trained control, restricted sedentary and restricted training. After AT protocol (60 minutes / day, 5 times / weeks, 10 weeks, intensity 50-60% of maximum speed). We observed that AT was effective in restoring ACh-induced vasodilatation in the thoracic aorta of restricted animals, this improvement was associated with normalization of NO concentration. The number of EPCs in bone marrow was reduced in trained control group compared to sedentary. The AT restored the functional capacity in vitro of circulating and bone marrow-derived EPCs in restricted group, which may be associated to the normalization of the number of senescent EPCs in bone marrow in these animals. Therefore, beneficial TA-induced adaptations in restricted animals in utero provide evidence that AT may be a non-pharmacological approach to reverse the negative adaptations in IUGR-induced vasculature and CPEs.
- ItemSomente MetadadadosMesenchymal stem cells and chronic renal artery stenosis(Amer Physiological Soc, 2016) Oliveira-Sales, Elizabeth Barbosa de [UNIFESP]; Boim, Mirian Aparecida [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Renal artery stenosis is the main cause of renovascular hypertension and results in ischemic nephropathy characterized by inflammation, oxidative stress, microvascular loss, and fibrosis with consequent functional failure. Considering the limited number of strategies that effectively control renovascular hypertension and restore renal function, we propose that cell therapy may be a promising option based on the regenerative and immunosuppressive properties of stem cells. This review addresses the effects of mesenchymal stem cells (MSC) in an experimental animal model of renovascular hypertension known as 2 kidney-1 clip (2K-1C). Significant benefits of MSC treatment have been observed on blood pressure and renal structure of the stenotic kidney. The mechanisms involved are discussed.
- ItemSomente MetadadadosMesenchymal stem cells and chronic renal artery stenosis(Amer Physiological Soc, 2016) Oliveira-Sales, Elizabeth B. [UNIFESP]; Boim, Mirian A. [UNIFESP]Renal artery stenosis is the main cause of renovascular hypertension and results in ischemic nephropathy characterized by inflammation, oxidative stress, microvascular loss, and fibrosis with consequent functional failure. Considering the limited number of strategies that effectively control renovascular hypertension and restore renal function, we propose that cell therapy may be a promising option based on the regenerative and immunosuppressive properties of stem cells. This review addresses the effects of mesenchymal stem cells (MSC) in an experimental animal model of renovascular hypertension known as 2 kidney-1 clip (2K-1C). Significant benefits of MSC treatment have been observed on blood pressure and renal structure of the stenotic kidney. The mechanisms involved are discussed.
- ItemSomente MetadadadosRenovascular hypertension: effects of mesenchymal stem cells in the contralateral hypertensive kidney in rats(Hospital Clinicas, Univ Sao Paulo, 2016) de Oliveira-Sales, Elizabeth Barbosa [UNIFESP]; Varela, Vanessa Araujo [UNIFESP]; Maquigussa, Edgar [UNIFESP]; Borges, Fernanda Teixeira [UNIFESP]; Shimoura, Caroline Gusson [UNIFESP]; Gomes, Guiomar [UNIFESP]; Campos, Ruy Ribeiro [UNIFESP]; Boim, Mirian Aparecida [UNIFESP]Mesenchymal stem cells (MSC) induced neovascularization and improved renal morphology of the stenotic kidney in 2 kidneys-1 clip (2K-1C) model of renovascular hypertension. The present study evaluated the effects of MSC in the contralateral hypertensive kidney. Three weeks after left renal artery occlusion, MSC were injected into the tail vein of the 2K-1C rats. Renal function and morphology were analyzed in both kidneys. Labeled MSC were found in stenotic and contralateral kidneys. Hypertensive 2K-1C animals presented increased circulating levels of Angiotensin II (Ang II) and renin. MSC prevented the progressive increase of blood pressure and reduced circulating Ang II and renin levels. Stenotic kidney showed reduced renal plasma flow (RPF) and glomerular filtration rate (GFR), whereas the contralateral kidney had a tendency (p > 0.5) of reduction in GFR in spite of unchanged RPF. MSC treatment caused an improvement in GFR with no effect of on RPF in the stenotic kidney. Contralateral kidney showed increased diuresis and natriuresis that were even higher in MSC-treated animals, indicating that cell treatment improved the capacity of the contralateral kidney to excrete sodium. Contralateral kidney expressed higher levels of inflammatory cytokines (IL-6, TNF-) and signs of fibrosis, which were attenuated by MSC treatment. MSC treatment improved the stenotic kidney function, and it was also beneficial to the contralateral hypertensive kidney because it improved the morphology and preserved its capacity to excrete sodium.