Navegando por Palavras-chave "Dinâmica Mitocondrial"

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    Efeitos da aplicação de vesículas extracelulares em ratos com doença renal crônica por Nx5/6 submetidos ao exercício físico
    (Universidade Federal de São Paulo (UNIFESP), 2019-01-31) Luiz, Rafael Da Silva [UNIFESP]; Borges, Fernanda Teixeira [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The repairing effect of mesenchymal stromal cells (MSCs), especially their extracellular vesicles (VEs), has received much attention. Additionally, knowledge about physical exercise has been growing, but the effect of its association with potential repair therapies is still little explored. The objective of this study was to evaluate the effect of the association between exercise and VEs on renal function in rats submitted to Nx5/6. Wistar rats were divided into control groups (C), Exercise (E), Sedentary + Nx5/6 (NS), Exercise + Nx5/6 (NS), NS + VE (NSV) and NE + EV (ENV). The training was 60min/day of swimming, 5 days per week for 8 weeks and the VEs (100μg) were injected into the caudal vein every 10 days for 50 days, both after surgery. Our results showed that Nx5/6 increased mean arterial pressure, glomerulosclerosis and proteinuria, leading to a decrease in renal function (decreased creatinine clearance and increased plasma urea), weight gain and cardio-respiratory capacity, causing an increase in mortality. Physical exercise increased cardiorespiratory capacity, improved renal function, but not proteinuria, showed antioxidant action by inducing mitochondrial dynamics (increased fusion and decreased mitochondrial fission), which reduced the mortality of nephrectomized animals. Extracellular vesicles extracted from MSCs also increased cardio-respiratory capacity, improved kidney function and decreased mortality in nephrectomized animals. The association was beneficial, since it potentiated the effects induced by the VEs. Our results suggest that physical exercise and administration of extracellular vesicles extracted from MSCs exert protective and/or repairing effects on the kidney and decrease the mortality of nephrectomized animals, probably by distinct mechanisms that may be potentiated by each other.
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    Papel da fissão mitocondrial na resposta à privação alimentar em caenorhabditis elegans
    (Universidade Federal de São Paulo (UNIFESP), 2019-06-27) Nunes, Danilo Rosa [UNIFESP]; Cunha, Fernanda Marques Da [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To investigate the impact of the absence of mitochondrial fission on the response to bacterial deprivation in C. elegans. Methods: Wild type worms and drp-1 mutants were grown to adulthood and transferred to NGM plates containing (ad libi- tum condition –AL) or not containing (bacterial deprivation condition -BD) an E. coli lawn. Animals were collected after different time points and mitochondrial parameters such as morphology, oxygen consumption, citrate synthase activity and ATP synthase levels were evaluated. The impact of BD and drp-1 mutation on lifespan and health- span were also assessed. Results: drp-1 mutation did not affect BD-induced exten- sion of life span. BD attenuated the age-related deterioration of body movement in wild type worms but failed to do so in drp-1 mutants, suggesting that mitochondrial fission is necessary for the benefits of BD in the body muscles. Muscle mitochondria seem to be more elongated in drp-1 mutants when compared to wild type animals, and this parameter is not affected by 24 h BD in the studied strains. In spite of the lack of effect of BD on morphology, mitochondrial oxygen consumption rates in live worms was drastically reduced by the nutritional intervention in both strains. This de- crease in respiration is not due to decreased mitochondrial mass, as revealed by the quantification of citrate synthase activity as well as by western immunoblotting for ATP synthase. Conclusion: Decreased mitochondrial fission does not interfere in the effects of BD on life span and mitochondrial activity, but seems to impair the benefits afforded by BD on the age-induced deterioration of mobility.