Navegando por Palavras-chave "Calophyllum brasiliense"

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    Estudo fitoquímico da espécie vegetal Calophyllum brasiliense (Clusiaceae) visando a seleção de compostos com ação antiparasitária e anttumoral
    (Universidade Federal de São Paulo, 2015-01-10) Silva, Luciana Grus Marques da [UNIFESP]; Lago, João Henrique Ghilardi [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    This work consists of phytochemical study and evaluation of the biological activity of the methanol extract of the bark of the trunk and essential oils from leaves, twigs and bark of Calophyllum brasiliense (Clusiaceae). First the extract was evaluated for antiparasitic potential and showed high potential antitripanossoma. Thus, this statement was submitted to the partition in AcOEt and the organic phase obtained was subjected to evaluation of their potential antiparasitic, showing excellent antitripanossoma activity (IC50 = 13 mg / mL) but inactive when measured against L. (L). infantum. (IC50> 300 g / ml). Then, this material was submitted to chromatographic procedures such as column chromatography (silica normal phase and Sephadex LH-20) chromatography and preparative thin layer which allowed the isolation of two coumarins: calanolideo E1 (1) and calanolideo E2 (2), this is the first occurrence of these compounds in C. brasiliense. The structures of these compounds were established by spectroscopic and spectrometric methods such as mass spectrometry (MS) and nuclear magnetic resonance (NMR), including through two-dimensional experiments. Finally, coumarins isolated were tested against trypomastigotes of T. cruzi and showed IC50 values ​​of xxx / mL (1) and xxx g / ml (2).
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    Estudo químico e farmacológico das cascas do tronco de Calophyllum brasiliense (Clusiaceae)
    (Universidade Federal de São Paulo, 2013-03-05) Rea, Alexandre [UNIFESP]; Lago, João Henrique Ghilardi [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    In the present work, was conducted the chemical study of methanol extract from the stem barks of Calophyllum brasiliense (Clusiaceae). This extract was also evaluated for antiparasitic potential and displayed high antitripanossomal potential. Therefore, this extract was subjected to partition with EtOAc and the obtained organic phase was subjected to evaluation of antiparasitic potential, showing excellent antitripanossomal activity (IC50 = 22 g/mL) while the same phase was inactive when evaluated against Leishmania sp.. Thus, this material was submitted to chromatographic procedures such as column chromatography (normal phase silica gel and Sephadex LH-20) affording the isolation of coumarin 6-hydroxy-4-propyl-5(3-hydroxy-2-methyl-1-oxobutyl)-6”,6” dimethylpyran-[2”,3”:8,7]-benzopyran-2-one, known as soulamarin, and the first occurrence of this compound C. brasiliense. The structure of this substance was defined by spectroscopic and spectrometric methods such as mass spectrometry (MS) and nuclear magnetic resonance (NMR), including twodimensional experiments. Finally, the coumarin soulamarin has been tested against trypomastigotes forms of T. cruzi and showed IC50 value of 85.3 g/mL, similar than determined to positive control benznidazol (IC50 of 114.6 g/mL). However, this value is higher than that found for the EtOAc partition phase of MeOH crude extract, suggesting that may be present in this material other inactive compounds that can act synergistically when in mixture with coumarin soulamarin.