Navegando por Palavras-chave "Células tronco"
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- ItemAcesso aberto (Open Access)Avaliação do transplante de células-tronco precursoras de neurônios inibitórios na amígdala basolateral em camundongos tratados com cocaína na ansiedade e na preferência condicionada por lugar e suas correlações neurobiológicas(Universidade Federal de São Paulo (UNIFESP), 2017-06-29) Yokoyama, Thais Suemi [UNIFESP]; Longo, Beatriz Monteiro [UNIFESP]; Marinho, Eduardo Ary Villela [UNIFESP]; http://lattes.cnpq.br/0245964878412260; http://lattes.cnpq.br/0245964878412260; http://lattes.cnpq.br/4851041162128915; Universidade Federal de São Paulo (UNIFESP)Drug addiction is one of the main problems modern society, as it can affect several areas of society, economy and public health. Studies have shown that in addition to the pharmacological effects of drugs of abuse, the environmental context where the individual makes use of it is extremely important for the development of dependence on the substance with potential for abuse. Moreover, they showed the important involvement of the basolateral amygdala by promoting desire for the use of the drug only by exposure to environments previously associated with such use. In order to better understand this pathology, we used stem cell transplantation, an inhibitory interneuron from the medial ganglionic eminence (EGM), to modulate pathways related to chemical dependence and to analyze possible behavioral changes. Thus, our objective was to evaluate the effects of stem cell transplantation of inhibitory interneuron on anxiety behaviors, preference conditioned by cocaine-induced site and characterize possible neuroanatomic structures involved in the conditioned response. For this, we used GFP + mice to obtain EGM stem cells and the hosts were Swiss mice at 2 months of age. After 30 days of cell transplantation, the animals were exposed to the high cross labyrinth for anxiety analysis, the next day the animals were conditioned to cocaine and submitted to the postconditioning test to evaluate the preference conditioned by place. Our results showed that inhibitory precursor cell transplantation was not able to alter anxiety behavior, but it potentiated cocaine- induced preference. In relation to the expression of c-fos, we evaluated the basolateral amygdala, medial prefrontal cortex, tegmental ventral area, nucleus accumbens (core and shell) and dorsal striatum. The expression of c-fos in the structures evaluated was not altered by the presence of EGM cells, only a pharmacological alteration of cocaine. Taken together, our results suggest that neural precursor transplantation from EGM is not capable of altering anxiety behavior, but has influenced cocaine-induced site-based preference
- ItemAcesso aberto (Open Access)Caracterização molecular e funcional das células do endométrio de mulheres com endometriose pélvica(Universidade Federal de São Paulo (UNIFESP), 2016-04-27) Nogueira, Adriana Luckow Invitti [UNIFESP]; Girão, Manoel João Batista Castello [UNIFESP]; http://lattes.cnpq.br/0973903299568770; http://lattes.cnpq.br/2547404412649527; Universidade Federal de São Paulo (UNIFESP)The endometrium contains stem cells that possibly contribute to the regeneration of the functional layer during the mensal cycling. Different kinds of stem cells were isolated from endometrium and endometriotic implants. The monoclonal origin of endometriotic implants indicates that stem cells may have a role in the endometriosis pathogenesis. We isolated a mixed cells pool containing at least one stem cells population from healthy and endometriosis endometrium. These cells presented the markers CD90, CD73, CD105, CD44 and CD146, being able to differentiate into adipogenic, osteogenic and chondrogenic lineages and not responding to progesterone stimulus. The endometriosis stem cells expressed the CD34 naïve stem cells marker, presented higher proliferative rates than healthy ones and were able to form long lasting cells aggregates (spheroids) in tridimensional cultures. The endometrium with endometriosis had a unique stem cell population, reinforcing the hypothesis that stem cells play a key role in the endometriosis pathogenesis. 2. INTRODUCTION Stem cells are undifferentiated cells defined by their functional ability to self-renew and to differentiate into multiple cell lineages, as well as by plasticity and clonogenic potential. They were found abundantly in embryonic and fetal tissues and are also present in small amounts in the majority of the adult tissues; differing between each other only in the clonogenic and differentiation potential. Adult stem cells occur in niches that balance self-renewal with lineage selection and progression during tissue homeostasis
- ItemAcesso aberto (Open Access)Efeitos das células tronco adultas de medula óssea e do plasma rico em plaquetas na regeneração e recuperação funcional nervosa em um modelo de defeito agudo em nervo perfiférico em rato(Sociedade Brasileira de Ortopedia e Traumatologia, 2006-01-01) Braga Silva, Jefferson [UNIFESP]; Gehlen, Daniel; Roman, Javier A.; Menta, Caroline; Atkinson, Eduardo De Andrade; Machado, Denise Cantarelli; Viezzer, Christian; Barbosa, Gustavo Leivas; Baes, Cristiane Von Werne; Silva, Vinícius Duval; Costa, Jaderson Costa Da; Universidade Federal de São Paulo (UNIFESP); Pontifícia Universidade Católica do Rio Grande do Sul; Pontifícia Universidade Católica do Rio Grande do Sul Faculdade de Medicina; Pontifícia Universidade Católica do Rio Grande do Sul Instituto de Pesquisas BiomédicasOBJECTIVES: The effects of the use of bone marrow stem cells (MSC) and platelet-rich plasma (PRP) on peripheral nerves regeneration were assessed by using an established model of sciatic nerve regeneration in rats. METHODS: A 10-mm nervous defect was reconstructed by using a silicone tube filled with MSC, PRP or both. The control group received only the silicone tube. A fifth group was also set, in which the interval was reconstructed by using a dried segment of the nerve. Motor function was tested six weeks after surgery, by means of a gait test. After motor test, the rats were anesthetized, the sciatic nerve and the tube were dried, and the transmission electronic microscopy was performed. RESULTS: The quantitative analysis shows an improved functional recovery in MSC group compared to the other groups. Nervous regeneration was reported for MSC group by means of transmission electronic microscopy with an almost full recovery of the neural anatomy. CONCLUSION: Our results suggest that the use of MSC combined with tubing technique yields a satisfactory recovery of motor function and nervous regeneration.
- ItemAcesso aberto (Open Access)Gene transfer to primary corneal epithelial cells with an integrating lentiviral vector(Conselho Brasileiro de Oftalmologia, 2010-10-01) Oliveira, Lauro Augusto De [UNIFESP]; Kim, Charles; Sousa, Luciene Barbosa De [UNIFESP]; Schwab, Ivan R.; Rosenblatt, Mark I.; Universidade Federal de São Paulo (UNIFESP); University of California Department of Ophthalmology and Visual Science; Weill Cornell Medical College Department of OphthalmologyPURPOSE: To evaluate the transfer of heterologous genes carrying a Green Fluorescent Protein (GFP) reporter cassette to primary corneal epithelial cells ex vivo. METHODS: Freshly enucleated rabbit corneoscleral tissue was used to obtain corneal epithelial cell suspension via enzymatic digestion. Cells were plated at a density of 5×10³ cells/cm² and allowed to grow for 5 days (to 70-80% confluency) prior to transduction. Gene transfer was monitored using fluorescence microscopy and fluorescence activated cell sorter (FACS). We evaluated the transduction efficiency (TE) over time and the dose-response effect of different lentiviral particles. One set of cells were dual sorted by fluorescence activated cell sorter for green fluorescent protein expression as well as Hoechst dye exclusion to evaluate the transduction of potentially corneal epithelial stem cells (side-population phenotypic cells). RESULTS: Green fluorescent protein expressing lentiviral vectors were able to effectively transduce rabbit primary epithelial cells cultured ex vivo. Live cell imaging post-transduction demonstrated GFP-positive cells with normal epithelial cell morphology and growth. The transduction efficiency over time was higher at the 5th post-transduction day (14.1%) and tended to stabilize after the 8th day. The number of transduced cells was dose-dependent, and at the highest lentivirus concentrations approached 7%. When double sorted by fluorescence activated cell sorter to isolate both green fluorescent protein positive and side population cells, transduced side population cells were identified. CONCLUSIONS: Lentiviral vectors can effectively transfer heterologous genes to primary corneal epithelial cells expanded ex vivo. Genes were stably expressed over time, transferred in a dose-dependence fashion, and could be transferred to mature corneal cells as well as presumable putative stem cells.
- ItemAcesso aberto (Open Access)Marcadores de doença aterosclerótica em pacientes infectados pelo HIV sem tratamento antirretroviral.(Universidade Federal de São Paulo (UNIFESP), 2010-08-25) Silva, Érika Ferrari Rafael da [UNIFESP]; Lewi, David Salomao [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Introduction: After the introduction of antiretroviral therapy, aids has acquired an aspect of chronic disease and less deaths has occurred. The deaths related to aids are diminished but the proportion of deaths unrelated to aids,including cardiovascular disease are increasing, in part due to the side effects from the antiretroviral therapy. Objectives: To quantify the number of endothelial progenitor cells (EPC) and microparticles (MP) in HIV-infected naïve patients and compared with a control group. Besides the biochemical profile we evaluated subclinical atherosclerosis through carotid intimal medial thickening and endothelial function by flow mediated dilation. Methods: A case-control study, matched for age and gender, cross-sectional with blind analysis of the goals of biomarkers. Quantification of EPC and MP was done with flow cytometry, subclinical atherosclerosis and endothelial function by echo-Doppler with high resolution linear transducer. We included 68 subjects (35 HIV + and 33 controls) with a median age of 33 years, 80% were male and Caucasian. Results: The lipid profile differ between the groups for total cholesterol 156 mg / dL and 173 mg / dL (p = 0.02) and HDL-C 37 mg / dL and 47 mg / dL (p = 0.001) for case and control groups, respectively. The count of the CEP was lower in HIV-infected patients compared with control CD34 + / KDR + [0.02% vs. 0.09%, p = 0.045], but not to CD34 + / CD133 + and KDR + / CD133 +. The average number of platelet MP was similar between the groups 24 992 per mL of plasma in HIV-infected group and 26 642 mL plasma in control (p = 0.83). The counting of endothelial MP / mL of plasma differed between groups, being higher in HIV-infected group compared to control (1963 vs. 436, p = 0.003). The measurement of intimal medial thickening was similar between groups, but was found lower flow-mediated dilation [mean (SD)] in the HIV patients [9% (5%) versus 16% (10%), p = 0.03]. Conclusions: HIV + patients, before HAART had lower amounts of EPC and increased endothelial MP, along with endothelial dysfunction.