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- ItemAcesso aberto (Open Access)Determinação da soroprevalência da infecção pelo vírus da hepatite e em pacientes submetidos à hemodiálise em comparação a transplantados renais e doadores de sangue(Universidade Federal de São Paulo (UNIFESP), 2018-05-05) Sena, Anne de [UNIFESP]; Bellei, Nancy Cristina Junqueira [UNIFESP]; http://lattes.cnpq.br/1571196803842272; http://lattes.cnpq.br/2665388053873440Introduction: Hepatitis E virus (HEV) infection currently accounts for over 50% of acute hepatitis cases in endemic countries. There are cases of hepatic failure in patients with other underlying liver diseases or in women in the third trimester of pregnancy, as well as the chronic form of the disease, especially among solid organ transplant patients. Objective: To establish the seroprevalence of HEV infection in patients submitted to hemodialysis compared to renal transplants patients and healthy controls (blood donors) by detecting antiHEV IgG and IgM antibodies, and in the positive samples the presence of RNAHEV via realtime RTPCR. Methods: 500 blood donors of the Benefit Blood Collection Association, 257 patients undergoing hemodialysis, and 292 kidney transplant recipients from the Hospital of Rim and Hypertension were included in the study. Detection of antiHEV IgG and antiHEV IgM antibodies was performed using the Wantai immunoenzyme assay (Beijing Wantai Biological Pharmacy Enterprise, Beijing, China). Samples with positive antiHEV IgG were submitted to viral RNA extraction for subsequent HEVRNA screening by realtime RTPCR. Results: The seroprevalence of HEV infection in hemodialysis patients was 10.9% when compared to renal transplant recipients (15.8%) and blood donors (9.8%), thus considering age as a risk factor for patients renal transplant patients and patients submitted to hemodialysis presented an increase for the development of hepatitis E in relation to the control group. In none of the seropositive samples was HEV RNA identified. Conclusion: This is the first study of the literature evaluating the seroprevalence of HEV infection in patients submitted to hemodialysis in Brazil and the correlation of the three groups studied shows that age is an important risk factor for the development of the disease.
- ItemAcesso aberto (Open Access)Estudo de aloanticorpos anti-HLA e anti-neutrófilos em doadoras de sangue(Universidade Federal de São Paulo (UNIFESP), 2008-02-27) Lopes, Larissa Barbosa [UNIFESP]; Bordin, Jose Orlando [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Purpose: The aim of this study were to determine the prevalence and the specificity of anti-HLA, class I and II, antibodies and the prevalence of anti-neutrophil antibodies in Brazilians female blood donors and to assess the impact of excluding the positive multiparous blood donors from the blood donation. Methods: Serum samples from 350 Brazilians female blood donors, distributed according to the number of previous pregnancy (50 nuliparous, 100 one pregnancy, 100 two pregnancies,100 multiparous), has been examined to detect and to identify anti-HLA, class I and II, antibodies by enzyme-linked immunosorbent assay (ELISA), by LAT-M and LAT-1240 Kits (ONE LAMBDA, INC, EUA). Sixty-five multiparous female blood donors, between positive and negative by LAT-M, were tested by flow cytometry, by LABScreen Kit (ONE LAMBDA, INC, EUA), that provide the specificity of anti-HLA antibodies. Furthermore, the Granulocyte Agglutination (GAT) and Granulocyte Immunofluorescence (GIFT) Test were used to detect specific anti-neutrophil antibodies. Results: Anti-HLA antibodies, class I and/or II, have been found in 80 of 350 female blood donors (22.9%), from whom 37% were multiparous donors, 26% woman with two previous pregnancies, and 17% woman with one previous pregnancy. All the nuliparous blood donors were negative to anti-HLA antibodies. The difference among positive female blood donors in pregnancy groups was significant statistically in prevalence between nuliparous group and woman with at least one previous pregnancy (p < 0.001). The serum of positive female blood donors by LAT-M test were also tested by LAT-1240 Test and in seven women the anti- HLA antibodies can not be identified. Seventeen female blood donors, that were considered negative by LAT-M, were positive for anti-HLA antibodies by LABScreen. The GAT was applied to investigate the serum of negative female blood donors to anti- HLA. Two women of 264 female blood donors (0.8%) were positive to anti-neutrophil, from whom 1.6% were multiparous (one of 62) and 1.4% were women with two previous pregnancies (one of 71). The GIFT was also applied to negative female blood donors to anti-HLA and the women were positive to anti-neutrophil in four of 264 female blood donors (1.5%), from whom two multiparous of 62 (3.2%) and two of 71 (2.8%) women with two previous pregnancy. Conclusion: Anti-HLA antibodies have often been found in plasma of Brazilian female blood donors (22.9%), and are statistically associated with the number of pregnancy (p < 0,001). The percentage of positive women increases with xviii the number of pregnancy. Although the anti-neutrophil antibodies had less prevalence (1.9% of all women tested by GAT and GIFT), they are found in female blood donors. This value can be higher because the positive women to anti-HLA antibodies were not tested. The impact to exclude the positive multiparous blood donors to the blood donate is 4% of all blood donors. In conclusion, anti-HLA and anti-neutrophil antibodies are found in plasma of Brazilian female blood donors and they are considered risk factor to trigger TRALI in blood receptors with cognate antigen.
- ItemAcesso aberto (Open Access)High prevalence of hepatitis E virus antibodies in Sao Paulo, Southeastern Brazil: analysis of a group of blood donors representative of the general population(Elsevier Brazil, 2017) Passos-Castilho, Ana Maria [UNIFESP]; Reinaldo, Monica Renata [UNIFESP]; de Sena, Anne [UNIFESP]; Granato, Celso F. H. [UNIFESP]Brazil is a non-endemic country for hepatitis E virus (HEV) infection with seroprevalence from 1% to 4% in blood donors and the general population. However, data on seroprevalence of HEV in the country are still limited. This study evaluated the prevalence of past or present HEV infection in a group of blood donors representative of the general population of the city of Sao Paulo, Southeastern Brazil. Serum samples from 500 blood donors were tested from July to September 2014 by serological and molecular methods. Anti-HEV IgG antibodies were detected in 49 (9.8%) subjects and categorized age groups revealed an age-dependent increase of HEV seroprevalence. Among the anti-HEV IgG positive subjects, only 1 had anti-HEV IgM while none tested positive for HEV-RNA. The present data demonstrate a higher seroprevalence of anti-HEV IgG than previously reported in the region. (C) 2017 Sociedade Brasileira de Infectologia. Published by Elsevier Editora Ltda.
- ItemAcesso aberto (Open Access)Soroprevalência de anticorpos anti-SARS-CoV-2 em doadores de sangue em diferentes populações do Estado de São Paulo e correlação com aspectos sócio demográficos(Universidade Federal de São Paulo, 2023-02-14) Vale, Nayara Moya Rios do [UNIFESP]; Bonetti, Tatiana Carvalho de Souza [UNIFESP]; Latini, Flávia Roche Moreira; http://lattes.cnpq.br/4844851515888706; http://lattes.cnpq.br/1502827442410231Objetivos: O presente estudo teve como objetivo identificar a presença de anticorpos anti-SARS-CoV-2 em doadores de sangue, em diferentes períodos da pandemia, comparando as regiões da capital, metropolitana e interior do estado de São Paulo, assim como sua correlação com perfil sócio demográfico dos doadores. Material e Métodos: foram avaliadas amostras de soro de doadores de sangue quanto à presença de anticorpos anti-SARS-CoV-2 em três períodos da pandemia de COVID-19 em São Paulo, Brasil; junho de 2020, outubro de 2020 e fevereiro de 2021. As amostras foram obtidas a partir de soroteca do banco de sangue da Associação Beneficente de Coleta de Sangue (COLSAN). Resultados: A soroprevalência global de anticorpos anti-SARS-CoV-2 foi de 21,5% considerando os três períodos e todas as regiões analisadas. Entretanto, a soroprevalência global se apresentou crescente de acordo com o momento da coleta, uma vez que foi de 11,4% em junho de 2020, 22% em outubro de 2020 e 30,9% em fevereiro de 2021. Nos três períodos analisados a capital apresentou a maior prevalência de anticorpos, seguido pela região metropolitana e interior. A correlação da soroprevalência com o perfil sócio demográfico mostrou que indivíduos com nível de escolaridade maior (graduação) possuíram menores chances de apresentar anticorpos positivos para SARS-CoV-2, por outro lado, ser afrodescendentes e utilização de transporte coletivo demonstraram ser fatores de risco para presença de anticorpos anti-SARS-CoV-2. Doadores que eram dos grupos sanguíneos A e AB também apresentaram maior soroprevalência de anticorpos para SARS-CoV-2 comparado àqueles dos grupos O e B. Conclusão: A investigação da soroprevalência em doadores de sangue de diferentes regiões do estado de São Paulo demonstrou ser uma estratégia eficaz para esclarecer o comportamento viral e evidenciar informações relevantes sobre populações vulneráveis, a fim de impulsionar estratégias para o combate à pandemia.