Navegando por Palavras-chave "Arginine"
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- ItemAcesso aberto (Open Access)Effects of L-arginine oral supplements in pregnant spontaneously hypertensive rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2006-08-01) Moura, José Ricardo Sousa Ayres De [UNIFESP]; Sass, Nelson [UNIFESP]; Guimarães, Sérgio Botelho; Vasconcelos, Paulo Roberto Leitão De; Mattar, Rosiane [UNIFESP]; Kulay Jr, Luis [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Federal University of Ceará; UFCPURPOSE: To evaluate the effects of L-arginine oral supplementation in spontaneously hypertensive pregnant rats (SHR). METHODS: Thirty SHR and ten Wistar-EPM-1 virgin female rats were used in the study. Before randomization, females were caged with males of the same strain (3:1). Pregnancy was confirmed by sperm-positive vaginal smear (Day 0). Wistar-EPM-1 rats served as counterpart control (C-1). SHR rats were randomized in 4 groups (n=10): Group Control 2, non-treated rats; Group L-Arginine treated with L-arginine 2%; Group Alpha-methyldopa treated with Alpha-methyldopa 33mg/Kg; Group L-Arginine+Alpha-methyldopa treated with L-arginine 2%+Alpha-methyldopa 33mg/Kg. L-arginine 2% solution was offered ad libitum in drinking water and Alpha-methyldopa was administered by gavage twice a day during the length of pregnancy (20 days). Blood pressure was measured by tailcuff plethysmography on days 0 and 20. Body weight was measured on days 0, 10 and 20. Results were expressed as mean ± SD (Standard Deviation). One-Way ANOVA/Tukey (or Kruskal-Wallis/Dunn, as appropriate) was used for group comparisons. Statistical significance was accepted as p<0.05. RESULTS: There was no significant weight gain in isolated L-arginine treated SHR. Mean blood pressure decreased in L-arginine-treated SLR compared with untreated-SHR rats. CONCLUSION: L-arginine oral supplementation reduces blood pressure in spontaneously hypertensive rats during pregnancy.
- ItemSomente MetadadadosEnzimas do ciclo ornitina-arginina em trioanosomatideos do gênero Herpetomonas(Universidade Federal de São Paulo (UNIFESP), 1978) Yoshida, Nobuko [UNIFESP]; Camargo, Erney Plessmann [UNIFESP]
- ItemAcesso aberto (Open Access)L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2012-09-01) Taha, Murched Omar [UNIFESP]; Caricati-Neto, Afonso [UNIFESP]; Miranda-Ferreira, Regiane [UNIFESP]; Simões, Manuel de Jesus [UNIFESP]; Monteiro, Hugo Pequeno [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To investigate the effects of intravenous L-arginine (LG) infusion on liver morphology, function and proinflammatory response of cytokines during the early phase of ischemia-reperfusion injury (IRI). METHODS: Thirty rabbits were subjected to 60 minutes of hepatic ischemia and 120 minutes of reperfusion. An intravenous injection of saline or L-arginine was administered five minutes before the ischemia and five minutes before initiating the reperfusion and at the 55th and 115th minutes after the ischemia. Samples were collected for histological analysis of the liver and measurements of the serum AST, ALT and LDH and the cytokines IL-6 and TNF-alpha. RESULTS: It was observed a significant reduction of sinusoidal congestion, cytoplasmic vacuolization, infiltration of polymorphonuclear leukocyte, nuclear pyknosis, necrosis and steatosis in liver tissue, as well as AST, ALT and LDH after injection of LG in the ischemia (p <0.001). Lower levels of IL-6 and TNF-alpha were associated with LG infusion during ischemia. Higher levels these proteins were observed in animals receiving LG during reperfusion. CONCLUSION: L-arginine protects the liver against ischemia/reperfusion injury, mainly when is administered during the ischemic phase.
- ItemAcesso aberto (Open Access)Nanopartícula pH responsiva para administração oral de antiparasitários no tratamento de peixes ornamentais(Universidade Federal de São Paulo (UNIFESP), 2020-01-30) Patta, Ana Carolina Monge Fernandes [UNIFESP]; Mertins, Omar [UNIFESP]; Delgado, Patrick Mathews [UNIFESP]; http://lattes.cnpq.br/1954400993734545; http://lattes.cnpq.br/8704899939986920; http://lattes.cnpq.br/4702078949333472; Universidade Federal de São PauloStudies show that the global trade in ornamental fish has a high economic impact, as it involves buying and selling millions of specimens a year, generating thousands of jobs and revenues. Meanwhile, it increases the risk of cross-border spread of infectious diseases through the spread of live animals, with devastating consequences. The present study aimed to develop a strategy of innovative aspects in the treatment of endoparasites in ornamental fish, through the production, characterization and application of nanoparticles with specific molecular characteristics of biological interaction. These chitosan-N-arginine and alginate composite nanoparticles were developed as a carrier system for anthelmintic ivermectin and praziquantel drugs and with muco-adhesive and pH responsive characteristics as a function of the application medium. For the development and study, physicochemical techniques of characterization were applied and application studies of the composites containing drugs were performed by oral administration in adult ornamental fish Corydoras schwartzi, parasitized with nematodes and digenetic trematodes, for evaluation of endoparasitic treatment. Studies have shown that the particles have pH-dependent surface charge and colloidal structure characteristics. With in vivo experiments it was observed that the particles remained in the fish's gastrointestinal system for periods longer than eight hours after administration, favoring the attenuated release of the drugs and the efficacy of the treatments, which achieved a reduction of up to 95% of the fish intestinal parasites. Thus, it was evidenced that the particles were effective in transporting and releasing drugs to the target region, enabling an innovative and effective treatment. The studies also open perspectives for the use of fish as an in vivo biological model to evaluate the applicability of bioactive carrier systems.
- ItemAcesso aberto (Open Access)Polimorfismos da p53 em mulheres com e sem expressão de RNAm E6/E7 de HPV(Universidade Federal de São Paulo (UNIFESP), 2015-12-31) Chuery, Ana Carolina Silva [UNIFESP]; Speck, Neila Maria de Gois [UNIFESP]; Ribalta, Julisa Chamorro Lascasas [UNIFESP]; http://lattes.cnpq.br/9709032217978701; http://lattes.cnpq.br/8169544398769371; http://lattes.cnpq.br/4398898060637058; Universidade Federal de São Paulo (UNIFESP)Objetivo: avaliar a associação entre polimorfismos da p53 e expressão de RNAm E6/E7 de papilomavírus humano (HPV). Métodos: estudo transversal que analisou 175 amostras cervicais de mulheres de 16 a 69 anos de idade que realizaram o teste de RNAm E6/E7 de HPV (NucliSENS® EasyQ® HPV). As amostras foram divididas em três grupos: positivo (n=75), aquelas com expressão de RNAm E6/E7 de HPV e captura híbrida (CH) para HPV de alto risco positiva; negativo (n=52), aquelas sem expressão de RNAm E6/E7 de HPV e CH positiva; e controle (n=48), as sem expressão de RNAm E6/E7 de HPV e CH negativa. A pesquisa dos polimorfismos nos códons 11, 72 e 248 da p53 foi feita pelo método polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Resultados: a frequência de genótipo com homozigose arginina/arginina no códon 72 foi significantemente maior no grupo positivo (49,3%) do que nos grupos negativo (32,7%) e controle (20,8%, p=0,002*). A frequência do alelo arginina também foi significantemente maior no grupo positivo (67,3%) do que nos grupos negativo (53,8%) e controle (38,5%, p<0,001*). A razão de chances da homozigose arginina/arginina associar-se com expressão de RNAm E6/E7 de HPV (grupo positivo) em comparação aos outros grupos (negativo e controle) foi 2,633 vezes (intervalo de confiança de 95%: 1,399-4,954, p=0,003*). A frequência de homozigose arginina/arginina no códon 72 manteve-se significativamente mais frequente no grupo positivo em comparação aos outros grupos nas amostras de mulheres com idade ?30 anos. Não houve diferença estatisticamente significante na distribuição genotípica nem na frequência alélica dos polimorfismos nos códons 11 e 248 entre os grupos positivo, negativo e controle. Conclusão: a presença de homozigose arginina/arginina no códon 72 da p53 associou-se significantemente com expressão de RNAm E6/E7 de HPV.
- ItemSomente MetadadadosShort-term specialized enteral diet fails to attenuate malnutrition impairment of experimental open wound acute healing(Elsevier B.V., 2010-09-01) Alves, Claudia Cristina [UNIFESP]; Torrinhas, Raquel Susana; Giorgi, Ricardo; Brentani, Maria Mitzi; Logullo, Angela Flavia [UNIFESP]; Arias, Victor [UNIFESP]; Mauad, Thais; Ferraz da Silva, Luiz Fernando; Waitzberg, Dan L.; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Objective: We assessed the effect of enteral refeeding on the morphology, gene expression, and contraction of acute open wounds in previously malnourished rats using two different enteral diets.Methods: Adult male isogenic Lewis rats divided into two groups (eutrophic, n = 30; and previously malnourished, 12-15% body weight loss, n = 27) were subjected to cutaneous dorsal wounds and gastrostomy. Control rats received a standard oral diet (AIN-93M chow) plus enteral saline solution. Subject rats received chow plus a standard enteral diet or an enteral diet enriched with arginine and antioxidants. On post-trauma days 7 and 14, wound granulation tissue samples were collected for morphologic analysis using hematoxylin and eosin and picrosirius stain or immunohistochemistry slides and real-time polymerase chain reaction for collagen I and III gene expression. Wound contraction was also evaluated by comparing wound images from days 0,7, and 14.Results: Malnourished control rats had increased intensity and duration of wound inflammation, impaired increase of fibroblast cells contingent on post-trauma days 7 to 14, decreased expression of collagen III, and less wound contraction compared with eutrophic control rats. A specialized enteral diet did not improve wound healing of malnourished rats but did promote wound contraction at post-trauma day 7 in eutrophic rats.Conclusion: Short-term enteral refeeding, even with a specialized diet, failed to protect previously wounded malnourished rats from a prolonged inflammatory phase and impaired healing. (C) 2010 Elsevier Inc. All rights reserved.
- ItemSomente MetadadadosTGF-beta(1) expression in wound healing is acutely affected by experimental malnutrition and early enteral feeding(Wiley-Blackwell, 2014-10-01) Alves, Claudia Cristina; Torrinhas, Raquel Susana; Giorgi, Ricardo; Brentani, Maria Mitzi; Logullo, Angela Flavia [UNIFESP]; Waitzberg, Dan Linetzky; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Malnutrition is associated with the delay or failure of healing. We assessed the effect of experimental malnutrition and early enteral feeding with standard diet or diet supplemented with arginine and antioxidants on the levels of mRNA encoding growth factors in acute, open wound healing. Standardised cutaneous dorsal wounds and gastrostomies for enteral feeding were created in malnourished (M, n = 27) and eutrophic control (E, n = 30) Lewis male adult rats. Both M and E rats received isocaloric and isonitrogenous regimens with oral chow and saline (C), standard (S) or supplemented (A) enteral diets. On post-trauma day 7, mRNA levels of growth factor genes were analysed in wound granulation tissue by reverse transcription polymerase chain reaction (RT-PCR). M(C) rats had significantly lower transforming growth factor (TGF-(1)) mRNA levels than E(C) rats (258 +/- 083 versus 353 +/- 057, P < 001) and in comparison with M(S) and M(A) rats (466 +/- 249 and 461 +/- 211, respectively; P < 005). VEGF and KGF-7 mRNA levels were lower in M(A) rats than in E(A) rats (074 +/- 016 versus 125 +/- 066; and 107 +/- 045 versus 179 +/- 089, respectively; P 004), but did not differ from levels in E(C) and M(C) animals. in experimental open acute wound healing, previous malnutrition decreased local mRNA levels of TGF-(1) genes, which was minimised by early enteral feeding with standard or supplemented diets.