Navegando por Palavras-chave "Antifungal Agents"
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- ItemSomente MetadadadosAntifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil(Wiley, 2016) Figueiredo, Thais Pandini; de Lucas, Rosymar Coutinho; Cazzaniga, Rodrigo Anselmo; Franca, Carolina Nunes [UNIFESP]; Segato, Fernando; Taglialegna, Rafael; Maffei, Cláudia Maria LeiteCryptococcosis is a leading invasive fungal infection in immunocompromised patients. Considering the high prevalence and severity of these infections in immunocompromised patients attended at HC-FMRP-USP, the present research aimed to characterize the clinical isolates of Cryptococcus strains by biochemical and molecular methods and evaluate antifungal susceptibility of clinical isolates. Fifty isolates from 32 HIV-positive patients were obtained at HC-FMRP-USP. Most of the isolates (78.1%) were identified as C. neoformans, and 100% of C. neoformans and C. gattii strains were susceptible to amphotericin B, ketoconazole and fluconazole. All isolates were classified as serotype A (grubbii variety) by PCR and most of them were characterized in mating type MATa. PCR analysis of specific M13 microsatellite sequence revealed that VNI type was predominant among C. neoformans, while VGII was predominant among C. gattii. The strains did not show a significant resistance to the antifungals tested, and Canavanine-Glycine-Bromthymol Blue Agar (CGB) proved to be a reliable test presenting a good correlation with the molecular characterization. C. neoformans isolated from disseminated infections in the same patient showed molecular identity when different anatomical sites were compared
- ItemSomente MetadadadosInfecção e colonização por Candida spp, em cavidade oral de pacientes transplantados hepáticos: identificação das espécies e perfil de sensibilidade(Universidade Federal de São Paulo (UNIFESP), 2020-06-25) Sabadin, Clarice Elvira Saggin [UNIFESP]; Barbosa, Dulce Aparecida [UNIFESP]; Universidade Federal de São PauloIntroduction: Fungal infections are frequent after liver transplantation, and these microorganisms are responsible for a high rate of morbidity and mortality. Objective: To verify infection and oral colonization by Candida in liver transplant patients, to identify the species, to test the main antifungals and to verify associated factors. Methodology: Cross-sectional study carried out with 97 liver transplant patients from a hospital in southern Brazil, in which an oral clinical examination was performed at the beginning of the study and another after six months (collection "A" and collection "B"), to check for infection/colonization by Candida. A form was filled out with sociodemographic and clinical data, after oral material from all patients was collected with a sterile swab, seeded on plates with Sabouraud Dextrose agar culture medium with Chloramphenicol and incubated at 350 C for 48 hours. The samples were identified by sequencing the ITS (Internal transcribed spacer) region of the rDNA. The antifungal sensitivity tests were performed according to CLSI (Clinical and Laboratory Standards Institute) protocol M-27A4, and the broth microdilution technique was performed with the antifungals fluconazole, amphotericin B and micafungin. Results: Of the 97 patients investigated, 15.47% had candidiasis in one of the collections, with C. albicans being the prevalent species. A strain of C. albicans and one of C. tropicalis resistant to fluconazole were found. In the 82 patients who did not present oral candidiasis, colonization was verified in 61% of them in collection A and in 59.8% in collection B. The distribution of species found in collections A and B respectively were C. albicans 29 (58%) and 27 (55.1%), C. glabrata 8 (16%) and 9 (18.4%), C. tropicalis 5 (10%) and 3 (6.12%), C. dubliniensis 5 (10% ) and 8 (16.3%) and C. fermentatti 03 (6%) and 2 (4.1%). Regarding micafungin, all isolates were susceptible. For amphotericin B all suggestive of susceptibility. The levels of resistance to fluconazole in collections A and B respectively were C. albicans (6.9% and 7.4%), C. glrabrata (12.5% and 11.1%), C. dubliniensis (20% and 12.5%), C. tropicalis (20% and 33.3%). There was no statistically significant difference between candidiasis, colonization and resistance with the associated factors. Conclusion: Liver transplant patients have candidiasis and oral colonization by Candida, the main species identified was C. albicans. Eleven fluconazole-resistant Candida isolates were found.