Navegando por Palavras-chave "Anticonvulsant"

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    Anticonvulsant and GABA uptake inhibition properties of venom fractions from the spiders Parawixia bistriata and Scaptocosa raptoria
    (Swets Zeitlinger Publishers, 2002-09-01) Rodrigues, Marcelo Cairrão Araujo; Ribeiro, Alessandra Mussi; Pizzo, Andrea Baldocchi; Fontana, Andreia Cristina Karklin; Beleboni, Renê de Oliveira; Coutinho-Netto, Joaquim; Miranda, Antonio de [UNIFESP]; Santos, Wagner Ferreira dos; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
    In this article we describe an in vivo anticonvulsant effect from denatured crude venom and partially isolated fractions from two spiders: Parawixia bistriata and Scaptocosa raptoria. Intracerebroventricular injections of these venoms and fractions abolished rat convulsive tonic-clonic seizures induced by picrotoxin, bicuculline and pentylenetetrazole, and also, inhibited GABA uptake in synaptosomes of rat cerebral cortex. the venoms described in this work seems to be promising tools for the study of the GABAergic system, and may be a potential source for new anticonvulsant drugs.
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    A anticonvulsive fraction from Scaptocosa raptoria (Araneae : Lycosidae) spider venom
    (Elsevier B.V., 2004-11-23) Ribeiro, Alessandra Mussi; Miranda, Antonio [UNIFESP]; Gobbo-Netto, Leonardo; Lopes, Norberto Peporine; Santos, Wagner Ferreira dos; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
    Several spider neurotoxins are known to show highly selective effects on nervous tissues. Intracerebral injection into rats of spider venom from Scaptocosa raptoria, prevents seizures induced by convulsant agents. Injection of phenytoin (390 pmol/200 nl), muscimol (90 pmol/200 nl), baclofen (500 pmol/200 nl) into the substantia nigra (SN) pars reticulata, protected rats from convulsions evoked by unilateral focal injection of bicuculline into the area tempestas by 50, 80, and 100%, respectively. Denatured S. raptoria crude venom (4.6 mug, 2.3 mug, and 920 ng/200 nl), when administered into the SN, prevented seizures elicited by bicuculline in the area tempestas by 100, 100, and 87.5%, respectively. the injection into the SN of 160 ng/200 nl of fraction SrTx1 isolated from S. raptoria venom, reduced the magnitude of seizures. This fraction was rechromatographed affording fractions SrTx1.1, SrTx1.2 and SrTx1.3, and they were administered into the SN at doses of 100, 200, and 400 ng/200 nl respectively. Fraction SrTx1.3 protected 50, 85.7, and 100% of the animals against the seizures elicited by bicuculline injected into the area tempestas. This suggests that S. raptoria venom as well as its SrTx1.3 fraction, might be potential sources of new anticonvulsant drugs. (C) 2004 Elsevier Ireland Ltd. All rights reserved.