Navegando por Palavras-chave "Adjuvant"
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- ItemSomente MetadadadosCaracterização biofísica de vesículas catiônicas de dodab contendo C24:1 beta-glicosilceramida (βGlcCer)(Universidade Federal de São Paulo (UNIFESP), 2019-05-30) Martins, Leticia de Souza [UNIFESP]; Rozenfeld, Julio Henrique Kravcuks [UNIFESP]; http://lattes.cnpq.br/4232139220639334; http://lattes.cnpq.br/8580993475540515; Universidade Federal de São Paulo (UNIFESP)Objective: To characterize the structure of DODAB vesicles containing βGlcCer using differential scanning calorimetry (DSC), electron paramagnetic resonance (EPR) spectroscopy and fluorescence microscopy. Methods: Pure vesicles of DODAB, βGlcCer, and also mixed vesicles of DODAB plus βGlcCer were prepared from lipid films resulting from the evaporation of chloroform: methanol solutions. EPR, DSC and fluorescence microscopy experiments were performed using 5 mol%, 9 mol% and 16 mol% of βGlcCer in relation to the total lipid concentration. Results: It was shown that βGlcCer destabilized the gel phase of DODAB vesicles by decreasing the gel phase packing. Thus, βGlcCer induced a decrease in the phase transition temperature and cooperativity of DODAB vesicles, as seen in DSC thermograms. EPR spectra also showed that βGlcCer induced an increase in the order and / or rigidity of the DODAB fluid phase. Despite their different structures, a similar effect of loosening of the gel phase and stiffening of the fluid phase was observed when low cholesterol fractions were incorporated into the DODAB vesicles. Conclusion: βGlcCer is completely miscible with DODAB at all fractions tested, since no domains were observed by fluorescence microscopy or ESR spectra. Structural characterization of DODAB vesicles containing βGlcCer may be important for the development of novel immunotherapeutic tools such as vaccine adjuvants.
- ItemSomente MetadadadosCo-administration of alpha-GalCer analog and TLR4 agonist induces robust CD8(+) T-cell responses to PyCS protein and WT-1 antigen and activates memory-like effector NKT cells(Academic Press Inc Elsevier Science, 2016) Coelho-dos-Reis, Jordana G.; Huang, Jing; Tsao, Tiffany; Pereira, Felipe V. [UNIFESP]; Funakoshi, Ryota; Nakajima, Hiroko; Sugiyama, Haruo; Tsuji, MoriyaIn the present study, the combined adjuvant effect of 7DW8-5, a potent alpha-GalCer-analog, and monophosphoryl lipid A (MPLA), a TLR4 agonist, on the induction of vaccine-induced CD8(+) T-cell responses and protective immunity was evaluated. Mice were immunized with peptides corresponding to the CD8+ T-cell epitopes of a malaria antigen, a circumsporozoite protein of Plasmodium yoelff, and a tumor antigen, a Wilms Tumor antigen-1 (WT-1), together with 7DW8-5 and MPLA, as an adjuvant. These immunization regimens were able to induce higher levels of CD8+ T-cell responses and, ultimately, enhanced levels of protection against malaria and tumor challenges compared to the levels induced by immunization with peptides mixed with 7DW8-5 or MPLA alone. Co-administration of 7DW8-5 and MPLA induces activation of memory-like effector natural killer T (NKT) cells, i.e. CD44(+) CD62L(-) NKT cells. Our study indicates that 7DW8-5 greatly enhances important synergistic pathways associated to memory immune responses when co-administered with MPLA, thus rendering this combination of adjuvants a novel vaccine adjuvant formulation. (C) 2016 Elsevier Inc. All rights reserved.
- ItemSomente MetadadadosModulation of the type I hypersensitivity late phase reaction to OVA by Propionibacterium acnes-soluble polysaccharide(Elsevier B.V., 2008-12-22) Squaiella, Carla Cristina [UNIFESP]; Figueiredo Longhini, Ana Leda [UNIFESP]; Braga, Eleni Goncalves [UNIFESP]; Mussalem, Juliana Sekeres [UNIFESP]; Ananias, Renata Zeigler [UNIFESP]; Yendo, Tatiana Mina [UNIFESP]; Straus, Anita H. [UNIFESP]; Toledo, Marcos S. [UNIFESP]; Takahashi, Helio K. [UNIFESP]; Hirata, Izaura Yoshico [UNIFESP]; Longo-Maugeri, Ieda Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Late phase reaction (LPR) of immediate hypersensitivity is a Th2 response characterized by eosinophil recruitment and related to allergic asthma pathogenesis. Several strategies were developed trying to control the tissue damage observed in this reaction. Recently, we verified that killed Propionibacterium acnes (P. acnes), a Gram-positive bacillus, immunomodulated LPR in a murine model, potentiating or suppressing it depending on the treatment protocol used. However, the bacterium compounds responsible for this effect are not known, leading us to investigate if P acnes purified Soluble polysaccharide (PS) could be a major component involved on the modulation induced by the bacterium. Recently. we demonstrated that PS, like P. acnes, induces adjuvant effect on DNA vaccine, increases bone marrow dendritic cell precursors in vivo and its maturation in vitro, and modulates in vitro macrophage tumoricidal activity. Herein, we determined the chemical PS composition, which is mainly constituted by galactopyranose, ribopyranose, arabinopyranose, glucopyranose, ribofuranose and mannopyranose, and analyzed its capacity to modulate the immediate hypersensitivity in mice. Animals were subcutaneously implanted with coagulated hen's egg white (HEW) and 14 days later challenged with ovalbumin (OVA) in the footpad, developing a typical LPR after 24 h. Similarly to the whole bacterium, Th2 response to OVA was potentiated when PS was administered concomitantly to HEW implantation, by increase in footpad eosinophilia and IL4-producing spleen cells, and decrease in anti-OVA IgG2a titers and IL-12- or IFN-gamma-producing cells. On the other hand, the reaction was abrogated when HEW implantation was performed 1 week after PS-treatment, by decrease in footpad swelling, eosinophilia and anti-OVA IgG1 levels, and increase in IgG2a titers and IL-12-producing cells, These data suggest that PS seems to be the major P. acnes compound responsible for its effects on the modulation of immediate hypersensitivity reaction in mice. (C) 2008 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosPropionibacterium acnes induces an adjuvant effect in B-1 cells and affects their phagocyte differentiation via a TLR2-mediated mechanism(Elsevier Gmbh, Urban & Fischer Verlag, 2016) Gambero, Monica [UNIFESP]; Teixeira, Daniela [UNIFESP]; Butin, Liane [UNIFESP]; Ishimura, Mayari Eika [UNIFESP]; Mariano, Mario [UNIFESP]; Popi, Ana Flavia [UNIFESP]; Longo-Maugeri, Ieda Maria [UNIFESP]B-1 lymphocytes are present in large numbers in the mouse peritoneal cavity, as are macrophages, and are responsible for natural IgM production. These lymphocytes migrate to inflammatory foci and are also involved in innate immunity. It was also demonstrated that B-1 cells are able to differentiated into phagocytes (B-1CDP), which is characterized by expression of F4/80 and increased phagocytic activity. B-1 cell responses to antigens and adjuvants are poorly characterized. It has been shown that Propionibacterium acnes suspensions induce immunomodulatory effects in both macrophages and B-2 lymphocytes. We recently demonstrated that this bacterium has the ability to increase B-1 cell populations both in vitro and in vivo. P. acnes induces B-1CDP differentiation, increases the expression of TLR2, TLR4 and TLR9 and augments the expression of CD80, CD86 and CD40 in B-1 and B-1CDP cells. Because P. acnes has been shown to modulate TLR expression, in this study, we investigated the role of TLR2 and TLR4 in B-1 cell population, including B-1CDP differentiation and phagocytic activity in vitro and in vivo. Interestingly, we have demonstrated that TLR2 signaling could be involved in the increase in the B-1 cell population induced by P. acnes. Furthermore, the early differentiation of B-1CDP is also dependent of TLR2. It was also observed that TLR signals also interfere in the phagocytic ability of B-1 cells and their phagocytes. According to these data, it is clear that P. acnes promotes an important adjuvant effect in B-1 cells by inducing them to differentiate into B-1CDP cells and modulates their phagocytic functions both in vivo and in vitro. Moreover, most of these effects are mediated primarily via TLR2. These data reinforce the findings that such bacterial suspensions have powerful adjuvant properties. The responses of B-1 cells to exogenous stimulation indicate that these cells are important to the innate immune response. (C) 2016 Elsevier GmbH. All rights reserved.
- ItemSomente MetadadadosA prospective study showing an excellent response of patients with low-risk differentiated thyroid cancer who did not undergo radioiodine remnant ablation after total thyroidectomy(Future Sci Ltd, 2016) Janovsky, Carolina Castro Porto Silva [UNIFESP]; Maciel, Rui M. B. [UNIFESP]; Camacho, Cleber P. [UNIFESP]; Padovani, Rosalia P. [UNIFESP]; Nakabashi, Claudia C. [UNIFESP]; Yang, Ji H. [UNIFESP]; Malouf, Eduardo Z. [UNIFESP]; Ikejiri, Elza S. [UNIFESP]; Mamone, M. Conceicao O. C. [UNIFESP]; Wagner, Jairo; Andreoni, Danielle M. [UNIFESP]; Biscolla, Rosa Paula M. [UNIFESP]Objectives: To prospectively evaluate the outcome of patients with low-risk papillary thyroid carcinoma treated with total thyroidectomy (TT) who did not undergo radioiodine remnant ablation (RRA). Study Design: We prospectively followed up 57 patients