Navegando por Palavras-chave "óxido nítrico e apoptose"

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    Efeito da administração da n-acetilcisteína na reposição volêmica sobre a lesão renal induzida por choque hemorrágico em ratos
    (Universidade Federal de São Paulo (UNIFESP), 2014-08-27) Moreira, Miriam Aparecida [UNIFESP]; Martins, Jose Luiz Martins [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To evaluate the effect of N-acetylcysteine on renal injury due to controlled hemorrhagic shock in rats. Method: hemorrhagic shock was induced in Wistar rats by withdrawing blood to reach mean arterial blood pressure to 35 ± 5 mmHg for 60 minutes blood. After this period, the animals were distributed randomly into two groups according to treatment: Ringer lactate group (RL), resuscitated with Ringer's lactate 33mL/Kg and N-acetylcysteine group (NAC), resuscitated with Ringer's lactate and 150mg/Kg of N-acetylcysteine and 50% of blood drawn for both groups. A third group of animals (control group) was submitted of catheterization procedure without inducing shock. After fluid resuscitation, the animals were observed for 120 minutes and euthanized. Results: NAC attenuated renal dysfunction, indicated by lower plasma creatinine in the group of animals treated with NAC compared to the RL group (0.39±0.1 vs 2.9±1.6 mg/dL, P<0.0001). The animals treated with NAC had less oxidative stress with lower values of TBARS tissue, nitric oxide and superoxide anion when compared with the RL group (0.60± 0.05 vs 1.3±0.2 nmol/mL protein,.184.7±20 vs 381.3±93 nM and 4.3±0.4 vs 18.2 ± 3.1%, P<0.0001, respectively) and apoptosis observed by the lower expression of Bax, cytochrome c and caspase 3 proteins in animals treated with NAC as compared to animals of RL group (0,9±0.06 vs 7.1±1.3; 0.3±0.3 vs 17.8±2.8; 3.8±0.4 vs. 10.0±1.5%, P<0.0001; respectively). NAC attenuated the morphological changes that were induced by the shock and larger diameter of the glomeruli when compared to the other two groups. Conclusion: NAC associated with fluid resuscitation attenuated renal injury in rats previously submitted to controlled hemorrhagic shock. NAC promotes improvement of morphological and functional changes and decreased oxidative stress and apoptosis of the kidney.