Plant Proteinase Inhibitor BbCI Modulates Lung Inflammatory Responses and Mechanic and Remodeling Alterations Induced by Elastase in Mice

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dc.contributor.authorAlmeida-Reis, Rafael
dc.contributor.authorTheodoro-Junior, Osmar A.
dc.contributor.authorOliveira, Bruno T. M.
dc.contributor.authorOliva, Leandro V.
dc.contributor.authorToledo-Arruda, Alessandra C.
dc.contributor.authorBonturi, Camila Ramalho [UNIFESP]
dc.contributor.authorBrito, Marlon Vilela de [UNIFESP]
dc.contributor.authorLopes, Fernanda D. T. Q. S.
dc.contributor.authorPrado, Carla Maximo [UNIFESP]
dc.contributor.authorFlorencio, Ariana C.
dc.contributor.authorMartins, Milton A.
dc.contributor.authorOwen, Caroline A.
dc.contributor.authorLeick, Edna A.
dc.contributor.authorOliva, Maria Luiza Vilela [UNIFESP]
dc.contributor.authorTiberio, Iolanda F. L. C.
dc.coverageLondon
dc.date.accessioned2020-07-31T12:46:43Z
dc.date.available2020-07-31T12:46:43Z
dc.date.issued2017
dc.description.abstractBackground. Proteinases play a key role in emphysema. Bauhinia bauhinioides cruzipain inhibitor (BbCI) is a serine-cysteine proteinase inhibitor. We evaluated BbCI treatment in elastase-induced pulmonary alterations. Methods. C57BL/6 mice received intratracheal elastase (ELA group) or saline (SAL group). One group of mice was treated with BbCI (days 1, 15, and 21 after elastase instillation, ELABC group). Controls received saline and BbCI (SALBC group). After 28 days, we evaluated respiratory mechanics, exhaled nitric oxide, and bronchoalveolar lavage fluid. In lung tissue we measured airspace enlargement, quantified neutrophils, TNF alpha-, MMP-9-, MMP-12-, TIMP-1-, iNOS-, and eNOS-positive cells, 8-iso-PGF2 alpha, collagen,and elastic fibers in alveolar septa and airways. MUC-5-positive cells were quantified only in airways. Results. BbCI reduced elastase-induced changes in pulmonary mechanics, airspace enlargement and elastase-induced increases in total cells, and neutrophils in BALF. BbCI reduced macrophages and neutrophils positive cells in alveolar septa and neutrophils and TNF alpha-positive cells in airways. BbCI attenuated elastic and collagen fibers, MMP-9- and MMP-12-positive cells, and isoprostane and iNOS-positive cells in alveolar septa and airways. BbCI reduced MUC5ac-positive cells in airways. Conclusions. BbCI improved lung mechanics and reduced lung inflammation and airspace enlargement and increased oxidative stress levels induced by elastase. BbCI may have therapeutic potential in chronic obstructive pulmonary disease.en
dc.description.affiliationUniv Sao Paulo, Sch Med, Dept Med, Sao Paulo, SP, Brazil
dc.description.affiliationHarvard Med Sch, Brigham & Womens Hosp, Div Pulm & Crit Care Med, Boston, MA USA
dc.description.affiliationUniv Fed Sao Paulo, Dept Biochem, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biol Sci, Sao Paulo, SP, Brazil
dc.description.affiliationLovelace Resp Res Inst, Albuquerque, NM USA
dc.description.affiliationUnifespDepartment of Biochemistry, Federal University of São Paulo, São Paulo, SP, Brazil
dc.description.affiliationUnifespDepartment of Biological Sciences, Federal University of São Paulo, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFaculdade de Medicina da Universidade de Sao Paulo, Brazil
dc.description.sponsorshipIDFaculdade de Medicina da Universidade de Sao Paulo, Brazil: LIM-20-HC-FMUSP
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1155/2017/8287125
dc.identifier.citationBiomed Research International. London, v. , p. -, 2017.
dc.identifier.doi10.1155/2017/8287125
dc.identifier.fileWOS000399211200001.pdf
dc.identifier.issn2314-6133
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56324
dc.identifier.wosWOS:000399211200001
dc.language.isoeng
dc.publisherHindawi Ltd
dc.relation.ispartofBiomed Research International
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titlePlant Proteinase Inhibitor BbCI Modulates Lung Inflammatory Responses and Mechanic and Remodeling Alterations Induced by Elastase in Miceen
dc.typeinfo:eu-repo/semantics/article
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