Isolation and identification of angiotensin-like peptides from the plasma of the snake Bothrops jararaca
| dc.contributor.author | Borgheresi, RAMB | |
| dc.contributor.author | DalleLucca, J. | |
| dc.contributor.author | Carmona, E. | |
| dc.contributor.author | Picarelli, Z. P. | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.date.accessioned | 2016-01-24T11:40:30Z | |
| dc.date.available | 2016-01-24T11:40:30Z | |
| dc.date.issued | 1996-03-01 | |
| dc.description.abstract | Two distinct hypertensive peptides were purified and characterized from Bothrops jararaca (Bj) plasma incubated at pH 4, 37 degrees C, 24 hr. These peptides were active on rat and Bj blood pressure, on rat isolated uterus, on guinea-pig isolated ileum and on Bj isolated duodenum. At the releasing conditions no further activities were found for kininases, angiotensinases or angiotensin converting enzymes. the peptides were purified by ethanol/ether extraction, Sephadex G-25 gel filtration, semipreparative reverse-phase (C-18) HPLC and analytical (C-18) HPLC. the amino-acid sequences of the purified peptides corresponded to (Ile(5))AII and (Val(5)-Tyr(9))AI and their molecular masses were confirmed by mass spectrometry as 1046.6 and 1348.0 respectively. the presence oi those two angiotensins on Bj plasma may have some evolutionary significance since (Ile(5))AII is known as a mammalian angiotensin and (Val(5))AII as a non-mammalian one. | en |
| dc.description.affiliation | ESCOLA PAULISTA MED,DEPT BIOFIS,São Paulo,BRAZIL | |
| dc.description.affiliationUnifesp | ESCOLA PAULISTA MED,DEPT BIOFIS,São Paulo,BRAZIL | |
| dc.description.source | Web of Science | |
| dc.format.extent | 467-473 | |
| dc.identifier | http://dx.doi.org/10.1016/0305-0491(95)02072-1 | |
| dc.identifier.citation | Comparative Biochemistry and Physiology B-biochemistry & Molecular Biology. Oxford: Pergamon-Elsevier B.V., v. 113, n. 3, p. 467-473, 1996. | |
| dc.identifier.doi | 10.1016/0305-0491(95)02072-1 | |
| dc.identifier.issn | 0305-0491 | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/25562 | |
| dc.identifier.wos | WOS:A1996UC56400005 | |
| dc.language.iso | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation.ispartof | Comparative Biochemistry and Physiology B-biochemistry & Molecular Biology | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
| dc.subject | amino-acid sequences | en |
| dc.subject | angiotensins | en |
| dc.subject | Bothrops jararaca snake | en |
| dc.subject | identification | en |
| dc.subject | peptide formation | en |
| dc.subject | renin angiotensin system | en |
| dc.subject | plasma | en |
| dc.subject | vasoactive peptides | en |
| dc.title | Isolation and identification of angiotensin-like peptides from the plasma of the snake Bothrops jararaca | en |
| dc.type | info:eu-repo/semantics/article |