ERM Proteins Play Distinct Roles in Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi

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dc.citation.volume8
dc.contributor.authorFerreira, Eden R. [UNIFESP]
dc.contributor.authorBonfim-Melo, Alexis [UNIFESP]
dc.contributor.authorCordero, Esteban M.
dc.contributor.authorMortara, Renato A. [UNIFESP]
dc.coverageLausanne
dc.date.accessioned2020-09-01T13:21:16Z
dc.date.available2020-09-01T13:21:16Z
dc.date.issued2017
dc.description.abstractThe protozoan parasite Trypanosoma cruzi is the causative agent of Chagas' disease. In mammalian hosts, T. cruzi alternates between trypomastigote and amastigote forms. Additionally, trypomastigotes can differentiate into amastigotes in the extracellular environment generating infective extracellular amastigotes (EAs). Ezrin-radixin-moesin (ERM) are key proteins linking plasma membrane to actin filaments, the major host cell component responsible for EA internalization. Our results revealed that depletion of host ezrin and radixin but not moesin inhibited EAs invasion in HeLa cells. ERM are recruited and colocalize with F-actin at EA invasion sites as shown by confocal microscopy. Invasion assays performed with cells overexpressing ERM showed increased EAs invasion in ezrin and radixin but not moesin overexpressing cells. Finally, time-lapse experiments have shown altered actin dynamics leading to delayed EA internalization in ezrin and radixin depleted cells when compared to control or moesin depleted cells. Altogether, these findings show distinct roles of ERM during EAs invasion, possibly regulating F-actin dynamics and plasma membrane interplay.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUniv Mayor, Fac Ciencias, Ctr Genom & Bioinformat, Santiago, Chile
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCAPES
dc.description.sponsorshipCNPq fellowship
dc.description.sponsorshipIDFAPESP: 2011/51475-3
dc.description.sponsorshipIDFAPESP: 2012/25282-6
dc.description.sponsorshipIDCNPq: 302068/2016-3
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2017.02230
dc.identifier.citationFrontiers In Microbiology. Lausanne, v. 8, p. -, 2017.
dc.identifier.doi10.3389/fmicb.2017.02230
dc.identifier.fileWOS000415793400001.pdf
dc.identifier.issn1664-302X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58164
dc.identifier.wosWOS:000415793400001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Microbiology
dc.rightsAcesso aberto
dc.subjectTrypanosoma cruzien
dc.subjectextracellular amastigoteen
dc.subjectERM proteinsen
dc.subjectHost cell invasionen
dc.subjectactin cytoskeletonen
dc.titleERM Proteins Play Distinct Roles in Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzien
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