Immunomodulatory and neuroprotective effect of cryopreserved allogeneic mesenchymal stem cells on spinal cord injury in rats

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dc.citation.issue1
dc.citation.volume16
dc.contributor.authorRosado, Isabel Rodrigues
dc.contributor.authorCarvalho, Pablo Herthel de
dc.contributor.authorAlves, Endrigo Gabellini Leonel
dc.contributor.authorTagushi, Tatiane Malagoli
dc.contributor.authorCarvalho, Juliana Lott
dc.contributor.authorSilva, Juneo de Freitas
dc.contributor.authorLavor, Mário Sergio Lima de
dc.contributor.authorOliveira, Karen Maciel de [UNIFESP]
dc.contributor.authorSerakides, Rogéria
dc.contributor.authorGoes, Alfredo Miranda de
dc.contributor.authorMelo, Eliane Gonçalves de
dc.coverageRibeirao Preto
dc.date.accessioned2020-07-17T14:02:38Z
dc.date.available2020-07-17T14:02:38Z
dc.date.issued2017
dc.description.abstractThis study aimed to evaluate the immunomodulatory and neuroprotective effects of allogeneic and cryopreserved mesenchymal stem cells (MSCs) on spinal cord injury. A total of 120 rats were distributed into the following groups: negative control (NC) - without injury, positive control (PC) - with injury without treatment, and group treated with MSC (GMSC) - with injury and treated. Motor function was evaluated by the BBB test at 24, 48, and 72 h and at 8 and 21 postoperative days. Spinal cords were evaluated by histopathology and immunohistochemistry to determine the expression of CD68, NeuN, and GFAP. IL-10, TNF-alpha, IL-1 beta, TGF-beta, BDNF, GDNF, and VEGF expression was quantified by RT-PCR. The GMSC presented higher scores for motor function at 72 h and 8 and 21 days after injury, lower expression of CD68 at 8 days, and lower expression of GFAP at 21 days compared to the PC. In addition, higher expression of NeuN and lower degeneration of the white matter occurred at 21 days. The GMSC also showed higher expression of IL-10 24 h after injury, GDNF at 48 h and 8 days, and VEGF at 21 days. Moreover, lower expression of TNF-alpha was observed at 8 and 21 days and TGF-beta at 24 h and 21 days. There were no differences in the expression of IL-1 beta and BDNF between the GMSC and PC. Thus, cryopreserved MSCs promote immunomodulatory and neuroprotective effects in rats with spinal cord injury by increasing IL-10, GDNF, and VEGF expression and reducing TNF-alpha and TGF-beta expression.en
dc.description.affiliationUniv Uberaba, Dept Med Ver, Uberaba, MG, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Escola Med Vet, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Estadual Santa Cruz, Dept Ciencias Biol, Ilheus, BA, Brazil
dc.description.affiliationUniv Estadual Santa Cruz, Dept Ciencias Agr & Ambientais, Ilheus, BA, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biociencias, Santos, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biociencias, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipVeterinary School
dc.description.sponsorshipLaboratory of Molecular Biology of the Department of Immunology and Biochemistry of the Institute of Biological Sciences
dc.format.extent-
dc.identifierhttps://dx.doi.org/10.4238/gmr16019555
dc.identifier.citationGenetics And Molecular Research. Ribeirao Preto, v. 16, n. 1, p. -, 2017.
dc.identifier.doi10.4238/gmr16019555
dc.identifier.fileWOS000417359800018.pdf
dc.identifier.issn1676-5680
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54912
dc.identifier.wosWOS:000417359800018
dc.language.isoeng
dc.publisherFunpec-Editora
dc.relation.ispartofGenetics And Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectNeurologyen
dc.subjectCell therapyen
dc.subjectRegenerative medicineen
dc.subjectInflammationen
dc.titleImmunomodulatory and neuroprotective effect of cryopreserved allogeneic mesenchymal stem cells on spinal cord injury in ratsen
dc.typeinfo:eu-repo/semantics/article
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