Serum anti-phenolic glycolipid1 IgA correlates to IgM isotype in leprosy patients: a possible candidate for seroepidemiological surveys?

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dc.citation.issue3
dc.citation.volumev. 32
dc.contributor.authorMacedo, Alexandre C. de
dc.contributor.authorGuimaraes, Juliana A.
dc.contributor.authorRodrigues, Raphael O.
dc.contributor.authorAraujo, Thiago D. V.
dc.contributor.authorTavares, Clodis M.
dc.contributor.authorCabral, Paula B.
dc.contributor.authorMoraes-Pinto, Maria Isabel de [UNIFESP]
dc.contributor.authorNagao-Dias, Aparecida T.
dc.coverageHoboken
dc.date.accessioned2020-07-20T16:31:22Z
dc.date.available2020-07-20T16:31:22Z
dc.date.issued2018
dc.description.abstractObjectiveThe aim of this study was to compare serum anti-phenolic glycolipid-1 IgA, IgG, and IgM levels in leprosy patients and controls. MethodAnalysis of anti-PGL-1 IgA, IgG, or IgM in serum samples from multibacillary (MB, n=32) and paucibacillary (PB, n=22) leprosy patients, and in non-endemic controls (n=17), using an indirect enzyme-linked immunosorbent assay. ResultsA strong correlation between serum IgM and IgA isotypes was found (r=.745, P<.0001) in MB patients. A moderate correlation was found in all analyses in PB patients. A moderate agreement was found between anti-PGL1 IgA and IgM tests. Based on the ROC curves, the cut-off values were selected and the parameters of validation were calculated. Considering the clinical forms altogether, the diagnostic sensitivities were 50.0% for IgA, 22.2% for IgG, and 74.1% for IgM. The positive (VPP) and negative (VPN) predictive values were estimated for each isotype. For IgA, the VPP and VPN were, respectively, 100.0% (87.0%-100.0%en
dc.description.abstract95% confidence interval) and 38.7% (24.4%-54.5%)en
dc.description.abstractfor IgG, 100% (87.0%-100.0%) and 28.8% (17.8%-42.1%), respectivelyen
dc.description.abstractand for IgM, 95.2% (83.8%-99.4%) and 51.7% (32.5%-70.6%), respectively. ConclusionDespite the limiting factors, anti-PGL1 IgA correlates to IgM levels and it could be considered as a possible laboratorial tool to be also used, for instance, in serological follow-up studies.en
dc.description.affiliationUniv Fed Ceara UFC, Dept Clin Anal & Toxicol, Fac Pharm, Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Ceara, Dept Pharm, Fac Pharm, Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Alagoas, Sch Nursery & Pharm ESENFAR, Maceio, Brazil
dc.description.affiliationUniv Fed Ceara, Hosp Univ Walter Cantidio, Div Pharm, Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Div Pediat Infect Dis, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Div Pediat Infect Dis, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipMCTI/CNPq/MS-SCTIE
dc.description.sponsorshipIDMCTI/CNPq/MS-SCTIE: 403461/2012-0
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1002/jcla.22276
dc.identifier.citationJournal Of Clinical Laboratory Analysis. Hoboken, v. 32, n. 3, p. -, 2018.
dc.identifier.doi10.1002/jcla.22276
dc.identifier.fileWOS000427567700001.pdf
dc.identifier.issn0887-8013
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55912
dc.identifier.wosWOS:000427567700001
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofJournal Of Clinical Laboratory Analysis
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectIgAen
dc.subjectIgGen
dc.subjectIgMen
dc.subjectisotypesen
dc.subjectMycobacterium lepraeen
dc.subjectnative PGL1en
dc.subjectserum antibodiesen
dc.titleSerum anti-phenolic glycolipid1 IgA correlates to IgM isotype in leprosy patients: a possible candidate for seroepidemiological surveys?en
dc.typeinfo:eu-repo/semantics/article
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