Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil

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dc.citation.issue1
dc.citation.volume21
dc.contributor.authorCampana, Eloiza Helena [UNIFESP]
dc.contributor.authorXavier, Danilo Elias [UNIFESP]
dc.contributor.authorPetrolini, Fernanda Villas-Boas [UNIFESP]
dc.contributor.authorCordeiro-Moura, Jhonatha Rodrigo [UNIFESP]
dc.contributor.authorElmor de Araujo, Maria Rita
dc.contributor.authorGales, Ana Cristina [UNIFESP]
dc.coverageRio De Janeiro
dc.date.accessioned2020-07-31T12:46:49Z
dc.date.available2020-07-31T12:46:49Z
dc.date.issued2017
dc.description.abstractThe mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in Sao Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. 8-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed by PFGE. Carbapenems hydrolysis was not detected by carbapenemase assay in the carbapenem-resistant and cephalosporin-susceptible P. aueruginosa clinical isolates. OprD decreased expression was observed in all P. aeruginosa isolates by qRT-PCR. The outer membrane protein profile by SDS-PAGE suggested a change in the expression of the 46 kDa porin that could correspond to OprD porin. The isolates were clustered into 17 genotypes without predominance of a specific PFGE pattern. These results emphasize the involvement of multiple chromosomal mechanisms in carbapenem-resistance among clinical isolates of P. aeruginosa, alert for adaptation of P. aeruginosa clinical isolates under antimicrobial selective pressure and make aware of the emergence of an uncommon phenotype among P. aeruginosa clinical isolates. (C) 2016 Sociedade Brasileira de Infectologia. Published by Elsevier Editora Ltda.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP), Lab ALERTA, Disciplina Infectol, Sao Paulo, SP, Brazil
dc.description.affiliationHosp Benemerita Soc Portuguesa Beneficencia & Hos, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Laboratório ALERTA, Disciplina de Infectologia, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2010/12891-9
dc.format.extent57-62
dc.identifierhttp://dx.doi.org/10.1016/j.bjid.2016.10.008
dc.identifier.citationBrazilian Journal Of Infectious Diseases. Rio De Janeiro, v. 21, n. 1, p. 57-62, 2017.
dc.identifier.doi10.1016/j.bjid.2016.10.008
dc.identifier.issn1413-8670
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56382
dc.identifier.wosWOS:000394831200009
dc.language.isoeng
dc.publisherElsevier Brazil
dc.relation.ispartofBrazilian Journal Of Infectious Diseases
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGram-negative bacillien
dc.subjectCarbapenem resistanceen
dc.subjectOprD porinen
dc.titleCarbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazilen
dc.typeinfo:eu-repo/semantics/article
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