Purification of Anti-Interleukin-6 Monoclonal Antibody Using Precipitation and Immobilized Metal-Ion Affinity Chromatography

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dc.contributor.authorAntunes Pereira Bresolin, Iara Rocha [UNIFESP]
dc.contributor.authorLazzarotto Bresolin, Igor Tadeu [UNIFESP]
dc.contributor.authorPessoa, Adalberto
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:40:02Z
dc.date.available2016-01-24T14:40:02Z
dc.date.issued2015-02-01
dc.description.abstractMonoclonal antibodies (MAbs) have been widely used in immunodiagnostic and clinical tests, whose applications require a high level of purity. As a result, several stages of purification are required in the downstream processing of MAbs. the objective of this study was to recover and purify an anti-interleukin-6 MAb from cell culture supernatant by a combined method involving precipitation and immobilized metal-ion affinity chromatography (IMAC), aiming its subsequent application in diagnostic kits. Experimental results suggest that it is possible to purify MAbs by precipitation with ammonium sulphate at 20-40% saturation, followed by adsorption on IMAC with immobilized Zn(II). the MAbs were obtained with a purity of 100% and a purification factor of 24.2, proving the feasibility of the method.en
dc.description.affiliationUniv São Paulo, Dept Biochem & Pharmaceut Technol, BR-05508000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Exact & Earth Sci, BR-09972270 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Exact & Earth Sci, BR-09972270 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent191-202
dc.identifierhttp://dx.doi.org/10.1260/0263-6174.33.2.191
dc.identifier.citationAdsorption Science & Technology. Brentwood: Multi-science Publ Co Ltd, v. 33, n. 2, p. 191-202, 2015.
dc.identifier.doi10.1260/0263-6174.33.2.191
dc.identifier.issn0263-6174
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38739
dc.identifier.wosWOS:000352264600010
dc.language.isoeng
dc.publisherMulti-science Publ Co Ltd
dc.relation.ispartofAdsorption Science & Technology
dc.rightsAcesso restrito
dc.titlePurification of Anti-Interleukin-6 Monoclonal Antibody Using Precipitation and Immobilized Metal-Ion Affinity Chromatographyen
dc.typeArtigo
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