Membrane-mediated action of the endocannabinoid anandamide on membrane proteins: implications for understanding the receptor-independent mechanism

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dc.citation.volume7
dc.contributor.authorMedeiros, Djalma [UNIFESP]
dc.contributor.authorSilva-Goncalves, Laiz da Costa [UNIFESP]
dc.contributor.authorBrito da Silva, Annielle Mendes [UNIFESP]
dc.contributor.authordos Santos Cabrera, Marcia Perez
dc.contributor.authorArcisio-Miranda, Manoel [UNIFESP]
dc.coverageLondon
dc.date.accessioned2020-07-17T14:03:13Z
dc.date.available2020-07-17T14:03:13Z
dc.date.issued2017
dc.description.abstractEndocannabinoids are amphiphilic molecules that play crucial neurophysiological functions acting as lipid messengers. Antagonists and knockdown of the classical CB1 and CB2 cannabinoid receptors do not completely abolish many endocannabinoid activities, supporting the idea of a mechanism independent of receptors whose mode of action remains unclear. Here we combine gramicidin A (gA) single channel recordings and membrane capacitance measurements to investigate the lipid bilayer-modifying activity of endocannabinoids. Single channel recordings show that the incorporation of endocannabinoids into lipid bilayers reduces the free energy necessary for gramicidin channels to transit from the monomeric to the dimeric conformation. Membrane capacitance demonstrates that the endocannabinoid anandamide has limited effects on the overall structure of the lipid bilayers. Our results associated with the theory of membrane elastic deformation reveal that the action of endocannabinoids on membrane proteins can involve local adjustments of the lipid/protein hydrophobic interface. The current findings shed new light on the receptor-independent mode of action of endocannabinoids on membrane proteins, with important implications towards their neurobiological function.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Biofis, Lab Neurobiol Estrutural Func LaNEF, Sao Paulo, SP, Brazil
dc.description.affiliationFac Sao Bento, Curso Filosofia, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, IBILCE, Dept Quim & Ciencias Ambientais, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Biofis, Lab Neurobiol Estrutural Func LaNEF, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (Fapesp)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipCNPq
dc.description.sponsorshipCapes
dc.description.sponsorshipIDFAPESP: 2012/02065-0
dc.description.sponsorshipIDFAPESP: 2012/24259-0
dc.description.sponsorshipIDFAPESP: 2014/08372-7
dc.description.sponsorshipIDCNPq: 477780/2010-5
dc.description.sponsorshipIDCNPq: 142066/2014-1
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1038/srep41362
dc.identifier.citationScientific Reports. London, v. 7, p. -, 2017.
dc.identifier.doi10.1038/srep41362
dc.identifier.fileWOS000392742900002.pdf
dc.identifier.issn2045-2322
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55231
dc.identifier.wosWOS:000392742900002
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reports
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleMembrane-mediated action of the endocannabinoid anandamide on membrane proteins: implications for understanding the receptor-independent mechanismen
dc.typeinfo:eu-repo/semantics/article
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