Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy

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dc.contributor.authorAlmodin, Carlos Gilberto [UNIFESP]
dc.contributor.authorMinguetti-Camara, V. C.
dc.contributor.authorMeister, H.
dc.contributor.authorFerreira, JOHR
dc.contributor.authorFranco, R. L.
dc.contributor.authorCavalcante, A. A.
dc.contributor.authorRadaelli, MRM
dc.contributor.authorBahls, A. S.
dc.contributor.authorMoron, Antonio Fernandes [UNIFESP]
dc.contributor.author|Murta, Carlos Geraldo Viana [UNIFESP]
dc.contributor.institutionMaterbaby Reprod Humana & Genet
dc.contributor.institutionUniversidade Estadual de Maringá (UEM)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Fed Espirito Santo
dc.date.accessioned2016-01-24T12:37:11Z
dc.date.available2016-01-24T12:37:11Z
dc.date.issued2004-06-01
dc.description.abstractBACKGROUND: Many cancer survivors face infertility as a consequence of the aggressive treatment they must undergo. Cryopreservation of ovarian tissue before chemotherapy or radiotherapy may allow for tissue transplantation after the treatment, and restoration of fertility. We tested the potential of an orthotopic autografting of cryopreserved germinative tissue in female rabbits with ovarian failure following radiotherapy. METHODS: Ten adult multiparous female rabbits were randomly allocated into two groups, five in group I (control) and five in group II (transplant). All rabbits underwent right oophorectomy with cryopreservation of the germinative tissue, followed by sterilization of the remaining left ovary by radiotherapy. Later, group II rabbits received in the irradiated left ovary an implant of the frozen germinative tissue from the right ovary, whose small pieces were freely spread intracortically in a procedure we named 'intracortical sowing of germinative tissue' (ISGT). RESULTS: All group II rabbits conceived following spontaneous mating within 6 months of the transplant, whereas none of the remaining rabbits in group I had conceived up to 11 months after transplant. CONCLUSIONS: This study suggests that fertility can be restored in rabbits by sowing cortical tissue in a previously irradiated ovary. the clinical feasibility of this technique remains to be determined.en
dc.description.affiliationMaterbaby Reprod Humana & Genet, BR-87013230 Maringa, Parana, Brazil
dc.description.affiliationUniv Estadual Maringa, Hosp Univ, Div Anal Clin, BR-87200000 Maringa, Parana, Brazil
dc.description.affiliationUniv Estadual Maringa, Dept Med, BR-87200000 Maringa, Parana, Brazil
dc.description.affiliationUniv Estadual Maringa, Dept Ciencias Morfofisiol, BR-87200000 Maringa, Parana, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Obstet & Tocoginecol, BR-04905000 São Paulo, Brazil
dc.description.affiliationUniv Fed Espirito Santo, Dept Med, BR-29102000 Vitoria, ES, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Obstet & Tocoginecol, BR-04905000 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1287-1293
dc.identifierhttp://dx.doi.org/10.1093/humrep/deh246
dc.identifier.citationHuman Reproduction. Oxford: Oxford Univ Press, v. 19, n. 6, p. 1287-1293, 2004.
dc.identifier.doi10.1093/humrep/deh246
dc.identifier.issn0268-1161
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27769
dc.identifier.wosWOS:000221748200009
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofHuman Reproduction
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.subjectautograften
dc.subjectcryopreservationen
dc.subjectfertilityen
dc.subjectovaryen
dc.titleRecovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapyen
dc.typeinfo:eu-repo/semantics/article
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