A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes

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dc.citation.volumev. 9
dc.contributor.authorFlorentino, Pilar T. V.
dc.contributor.authorReal, Fernando
dc.contributor.authorOrikaza, Cristina Mary [UNIFESP]
dc.contributor.authorCunha, Julia P. C. da
dc.contributor.authorVitorino, Francisca N. L.
dc.contributor.authorCordero, Esteban M.
dc.contributor.authorSobreira, Tiago J. P.
dc.contributor.authorMortara, Renato Arruda [UNIFESP]
dc.coverageLausanne
dc.date.accessioned2020-07-20T16:31:00Z
dc.date.available2020-07-20T16:31:00Z
dc.date.issued2018
dc.description.abstractTrypanosoma cruzi is the etiologic agent of Chagas' disease. It is known that amastigotes derived from trypomastigotes in the extracellular milieu are infective in vitro and in vivo. Extracellular amastigotes (EAs) have a stage-specific surface antigen called Ssp-4, a GPI-anchored glycoprotein that is secreted by the parasites. By immunoprecipitation with the Ssp-4-specific monoclonal antibodies (mAb) 2C2 and 1D9, we isolated the glycoprotein from EAs. By mass spectrometry, we identified the core protein of Ssp-4 and evaluated mRNA expression and the presence of Ssp-4 carbohydrate epitopes recognized by mAb1D9. We demonstrated that the carbohydrate epitope recognized by mAb1D9 could promote host cell invasion by EAs. Although infectious EAs express lower amounts of Ssp-4 compared with less-infectious EAs (at the mRNA and protein levels), it is the glycosylation of Ssp-4 (identified by mAb1D9 staining only in infectious strains and recognized by galectin-3 on host cells) that is the determinant of EA invasion of host cells. Furthermore, Ssp-4 is secreted by EAs, either free or associated with parasite vesicles, and can participate in host-cell interactions. The results presented here describe the possible role of a carbohydrate moiety of T. cruzi surface glycoproteins in host cell invasion by EA forms, highlighting the potential of these moieties as therapeutic and vaccine targets for the treatment of Chagas' disease.en
dc.description.affiliationUniv Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, Brazil
dc.description.affiliationINSERM, Inst Cochin, U1016, Paris, France
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationButantan Inst, Immune Response & Cell Signaling CeTICS, Ctr Toxins, Special Lab Cell Cycle, Sao Paulo, Brazil
dc.description.affiliationUniv Mayor, Ctr Genom & Bioinformat, Fac Ciencias, Santiago, Chile
dc.description.affiliationPurdue Univ, Bindley Biosci Ctr, W Lafayette, IN USA
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2011/51475-3
dc.description.sponsorshipIDFAPESP: 2012/23150-5
dc.description.sponsorshipIDCNPq: 302068/2016-3
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2018.00693
dc.identifier.citationFrontiers In Microbiology. Lausanne, v. 9, p. -, 2018.
dc.identifier.doi10.3389/fmicb.2018.00693
dc.identifier.fileWOS000429562900001.pdf
dc.identifier.issn1664-302X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55639
dc.identifier.wosWOS:000429562900001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTrypanosoma cruzien
dc.subjectextracellular amastigotesen
dc.subjectSsp-4 glycoproteinen
dc.subjectgalectin-3en
dc.titleA Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotesen
dc.typeinfo:eu-repo/semantics/article
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