The role of hemocytes in the immunity of the spider Acanthoscurria gomesiana

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dc.contributor.authorFukuzawa, Aline H.
dc.contributor.authorVellutini, Bruno C.
dc.contributor.authorLorenzini, Daniel M.
dc.contributor.authorSilva, Pedro I.
dc.contributor.authorMortara, Renato A. [UNIFESP]
dc.contributor.authorSilva, Jose M. C. da
dc.contributor.authorDaffre, Sirlei
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:49:22Z
dc.date.available2016-01-24T13:49:22Z
dc.date.issued2008-01-01
dc.description.abstractInvertebrates protect themselves against microbial infection through cellular and humoral immune defenses. Since the available information on the immune system of spiders is scarce, the main goat of the present study was to investigate the role of hemocytes and antimicrobial peptides (AMPs) in defense against microbes of spider Acanthoscurria gomesiana. We previously described the purification and characterization of two AMPs from the hemocytes of naive spider A. gomesiana, gomesin and acanthoscurrin. Here we show that 57% of the hemocytes store both gomesin and acanthoscurrin, either in the same or in different granules. Progomesin labeling in hemocyte granules indicates that gomesin is addressed to those organelles as a propeptide. in vivo and in vitro experiments showed that lipopolysaccharide (LPS) and yeast caused the hemocytes to migrate. Once they have reached the infection site, hemocytes may secrete coagulation cascade components and AMPs to cell-free hemolymph. Furthermore, our results suggest that phagocytosis is not the major defense mechanism activated after microbial challenge. Therefore, the main reactions involved in the spider immune defense might be coagulation and AMP secretion. (C) 2007 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, BR-05508900 São Paulo, Brazil
dc.description.affiliationInst Butantan, Lab Toxinol Aplicada, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent716-725
dc.identifierhttp://dx.doi.org/10.1016/j.dci.2007.11.002
dc.identifier.citationDevelopmental and Comparative Immunology. Oxford: Pergamon-Elsevier B.V., v. 32, n. 6, p. 716-725, 2008.
dc.identifier.doi10.1016/j.dci.2007.11.002
dc.identifier.issn0145-305X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30279
dc.identifier.wosWOS:000254781600013
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofDevelopmental and Comparative Immunology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectspideren
dc.subjectimmunityen
dc.subjecthemocytesen
dc.subjectantimicrobial peptidesen
dc.subjectsecretionen
dc.subjectcoagulationen
dc.titleThe role of hemocytes in the immunity of the spider Acanthoscurria gomesianaen
dc.typeinfo:eu-repo/semantics/article
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