Effect of estrogen on intracellular signaling pathways linked to activation of M-2- and M-3-muscarinic acetylcholine receptors in the rat myometrium

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dc.contributor.authorAbdalla, Fernando M. F.
dc.contributor.authorAbreu, Lygia C. [UNIFESP]
dc.contributor.authorPorto, Catarina S. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInst Butantan
dc.date.accessioned2016-01-24T12:31:01Z
dc.date.available2016-01-24T12:31:01Z
dc.date.issued2000-02-25
dc.description.abstractThe estrogen treatment of adult female rats induces an increase in myometrium sensitivity to cholinergic agonists and in this tissue the presence of M-2- and M-3-muscarinic acetylcholine (mACh) receptor was shown. We now report the effect of estrogen on intracellular signaling pathways linked to activation of M-2- and M-3-mACh receptor subtypes. the intracellular cyclic AMP accumulation and [H-3]-inositol phosphates content were measured in myometrium strips from rats in estrus (control) and estradiol-treated rats (12.5 mu g/100 g body weight, sc, 24 h before experiments) (the plasma estradiol level was 30.9 +/- 3.5 pg/ml and 119.3 +/- 14.1 pg/ml from control and estrogen-treated rats, respectively). Estrogen treatment increased 2.5-fold the intracellular cyclic AMP accumulation induced by 10 mu M forskolin. the effects of muscarinic agonist and antagonists on cyclic AMP accumulation were tested. Carbachol reduced the forskolin-induced intracellular cyclic AMP content, 3.0 and 10.5-fold, in myometrium from control and estradiol-treated rats, respectively. This inhibitory effect failed to occur when carbachol was incubated in the presence of methoctramine. Carbachol also induced increase on total [H-3]-inositol phosphates accumulation in myometrium from estradiol-treated rats when compared with control rats. This effect was reversed by pfHHSiD. These studies suggest the modulation by estrogen of intracellular signaling pathways linked to activation of M-2- and M-3-mACh receptors in the rat myometrium. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Expt Endocrinol Sect, BR-04044020 São Paulo, Brazil
dc.description.affiliationInst Butantan, Pharmacol Lab, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Expt Endocrinol Sect, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent17-24
dc.identifierhttps://dx.doi.org/10.1016/S0303-7207(99)00259-2
dc.identifier.citationMolecular and Cellular Endocrinology. Clare: Elsevier Sci Ireland Ltd, v. 160, n. 1-2, p. 17-24, 2000.
dc.identifier.doi10.1016/S0303-7207(99)00259-2
dc.identifier.issn0303-7207
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26252
dc.identifier.wosWOS:000086131300003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMolecular and Cellular Endocrinology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectestrogenen
dc.subjectmuscarinic acetylcholine receptor subtypesen
dc.subjectcyclic AMPen
dc.subjectinositol phosphateen
dc.subjectmyometriumen
dc.titleEffect of estrogen on intracellular signaling pathways linked to activation of M-2- and M-3-muscarinic acetylcholine receptors in the rat myometriumen
dc.typeinfo:eu-repo/semantics/article
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