Plasma Lipidomic Fingerprinting to Distinguish among Hepatitis C-related Hepatocellular Carcinoma, Liver Cirrhosis, and Chronic Hepatitis C using MALDI-TOF Mass Spectrometry: a Pilot Study

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dc.contributor.authorPassos-Castilho, Ana Maria [UNIFESP]
dc.contributor.authorLo Turco, Edson [UNIFESP]
dc.contributor.authorFerraz, Maria Lucia [UNIFESP]
dc.contributor.authorMatos, Carla [UNIFESP]
dc.contributor.authorSilva, Ivonete [UNIFESP]
dc.contributor.authorParise, Edison [UNIFESP]
dc.contributor.authorPilau, Eduardo
dc.contributor.authorGozzo, Fabio
dc.contributor.authorGranato, Celso [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniv Maringa
dc.date.accessioned2018-06-15T17:58:47Z
dc.date.available2018-06-15T17:58:47Z
dc.date.issued2015-03-01
dc.description.abstractBackground & Aims: Hepatitis C (HC) is a major cause of hepatocellular carcinoma (HCC), and a late diagnosis is the main factor for the poor survival of patients. There is an urgent need for identifying sensitive and specific biomarkers for HCC diagnosis. In the present study, plasma lipid patterns of patients with HCHCC, HC-liver cirrhosis (LC), and chronic HC (CHC) were assessed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS).Methods. Plasma samples of 25 patients with HC-HCC, 15 patients with HC-LC, and 25 patients with CHC were evaluated by MALDI-MS using a Q-ToF premier (Synapt) mass spectrometer (Waters, Manchester, UK) equipped with a 200-Hz solid-state laser in the mass range between m/z (mass-to-charge ratio) of 700-1200.Results. A total of 2205 ions were initially obtained and 7 ions (m/z) were highlighted as corresponding to the most important lipids to differentiate HCC patients from LC and CHC patients. The specific lipidomic expression signature generated resulted in an overall predictive accuracy of 93% of HC-HCC and HC-LC, and 100% of HC-HCC and CHC. The 7-peak algorithm distinguished HCC from LC with a sensitivity of 96% and a specificity of 87%, and HCC from CHC with both sensitivity and specificity of 100%.Conclusion. MALDI-MS-specific signature peaks accurately distinguished patients with HC-HCC from those with FIC-LC and CHC. The results indicate the potential of MALDI-MS and the selected peaks to improve HCC surveillance in patients with viral C cirrhosis and chronic hepatitis C.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Med, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Surg, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Gastroenterol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Chem, Campinas, SP, Brazil
dc.description.affiliationUniv Maringa, Dept Chem, Maringa, Parana, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Med, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Surg, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Gastroenterol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2013/03701-0
dc.format.extent43-49
dc.identifierhttp://dx.doi.org/10.15403/jgld.2014.1121.pas
dc.identifier.citationJournal Of Gastrointestinal And Liver Diseases. Cluj-napoca: Medical Univ Press, v. 24, n. 1, p. 43-49, 2015.
dc.identifier.doi10.15403/jgld.2014.1121.pas
dc.identifier.issn1841-8724
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/44367
dc.identifier.wosWOS:000350844200010
dc.language.isoeng
dc.publisherMedical Univ Press
dc.relation.ispartofJournal Of Gastrointestinal And Liver Diseases
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjecthepatocellular carcinomaen
dc.subjecthepatitis Cen
dc.subjectlipidomicsen
dc.subjectfingerprintsen
dc.subjectlipidsen
dc.subjectMALDI-MSen
dc.titlePlasma Lipidomic Fingerprinting to Distinguish among Hepatitis C-related Hepatocellular Carcinoma, Liver Cirrhosis, and Chronic Hepatitis C using MALDI-TOF Mass Spectrometry: a Pilot Studyen
dc.typeinfo:eu-repo/semantics/article
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