Determination of testicular function in adolescents with varicocoele - a proteomics approach

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dc.citation.issue3
dc.citation.volume4
dc.contributor.authorDel Giudice, Paula Toni [UNIFESP]
dc.contributor.authorBelardin, Larissa Berloffa [UNIFESP]
dc.contributor.authorCamargo, M. [UNIFESP]
dc.contributor.authorZylbersztejn, Daniel Suslik [UNIFESP]
dc.contributor.authorCarvalho, V. M.
dc.contributor.authorCardozo, K. H. M. [UNIFESP]
dc.contributor.authorBertolla, Ricardo Pimenta [UNIFESP]
dc.contributor.authorCedenho, Agnaldo Pereira [UNIFESP]
dc.coverageHoboken
dc.date.accessioned2020-07-22T13:22:56Z
dc.date.available2020-07-22T13:22:56Z
dc.date.issued2016
dc.description.abstractThe goal of this study was to determine seminal plasma biomarkers of testicular function in adolescents with varicocoele and to verify enriched gene ontology terms associated to these differential proteomes. An observational study was carried out in an academic research environment. A total of 77 adolescent patients were recruited from a local public school, of which 23 were without varicocoele and with normal semen analysis (control group), 37 were with varicocoele and normal semen (VNS) parameters, and 17 were with varicocoele and altered semen (VAS) parameters. Two semen collections were provided with a 1-week interval, after 2-5days of ejaculatory abstinence. Seminal plasma proteins were identified and quantified utilizing a label-free shotgun proteomics approach, generating (i) proteins differentially expressed in each group (control, VNS, and VAS) and putative biomarkers using multivariate statistics followed by discriminant analysis. Confirmatory analysis was performed for two proteins by western blotting. Enriched biological processes and molecular functions were determined using gene ontology analysis. In total, 541 proteins were identified and quantified: 108 exclusive or overexpressed in controls, 26 in the VNS group, and 13 in the VAS group. The suggested biomarkers are Cab45/SDF4 (Q9BRK5), protein lefty-1 (), DNase I (), PAP2-alpha (), IBP-7 (), HDC (), and CRISP-3 (). Western blotting results showed that Cab45 was significantly underexpressed in both varicocoele groups, and CRISP-3 was significantly overexpressed in seminal plasma of adolescents with VAS. In conclusion, specific biomarkers of spermatogenesis and homeostasis are observed in adolescents without varicocoele, and the presence of a palpable varicocoele progressively shifts these adolescents toward initially an immune response, and finally toward a chronic inflammatory profile. This shift is accompanied by decreased semen quality.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Surg, Human Reprod Sect, Div Urol, BR-04039060 Sao Paulo, SP, Brazil
dc.description.affiliationHosp Sao Paulo, Sao Paulo, Brazil - Fleury Grp, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo Research Foundation [FAPESP 2014/17185-6, FAPESP 2012/15039-7, FAPESP 2014/11636-6]
dc.description.sponsorshipNational Council for Scientific and Technological Development of Brazil [CNPq 472941/2012-7, CNPq 306616/2013-0]
dc.description.sponsorshipFleury Group
dc.description.sponsorshipCNPq
dc.format.extent447-455
dc.identifierhttp://dx.doi.org/10.1111/andr.12174
dc.identifier.citationAndrology. Hoboken, v. 4, n. 3, p. 447-455, 2016.
dc.identifier.doi10.1111/andr.12174
dc.identifier.issn2047-2919
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55952
dc.identifier.wosWOS:000375881200012
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofAndrology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectadolescenten
dc.subjectfunctional enrichmenten
dc.subjectproteomicsen
dc.subjectseminal plasmaen
dc.subjectvaricocoeleen
dc.titleDetermination of testicular function in adolescents with varicocoele - a proteomics approachen
dc.typeinfo:eu-repo/semantics/article
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