Effects of Glucose Deprivation or Glucose Instability on Mesangial Cells in Culture

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dc.contributor.authorSantos, Carla de Lima e [UNIFESP]
dc.contributor.authorArnoni, Carine P. [UNIFESP]
dc.contributor.authorSchor, Nestor [UNIFESP]
dc.contributor.authorBoim, Mirian A. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:52:08Z
dc.date.available2016-01-24T13:52:08Z
dc.date.issued2009-01-01
dc.description.abstractMesangial cell ( MC) abnormalities play a central role in diabetic nephropathy. Variations in plasma glucose levels may contribute to MC dysfunction. This study evaluated the effects of glucose deprivation or fluctuations on MC viability, glucose uptake, and mesangial matrix production. the expression levels of fibronectin and glucose transporters (GLUT-1 and GLUT-4) were determined. MCs were exposed to normal (NG, 10 m M), low (LG, 1 m M) or high (HG, 30 m M) glucose concentrations for 1, 4, 12, 24 and 72 h. Glucose oscillation was achieved by alternating the glucose concentration (LG, NG or HG) in the medium every 8, 12, and 4 h over a total of 24 h. LG induced a significant increase (90%) in glucose uptake dependent of GLUT-1, which was not followed by alteration in fibronectin expression. Fluctuations in glucose levels induced a rise in glucose uptake also mediated by GLUT-1. Fibronectin did not change in any oscillation group. Results suggest that, in spite of a rise in glucose uptake by MCs under low glucose availability, or exposed to glucose fluctuations, mesangial matrix overproduction did not occur. Thus, neither glucose deprivation nor glucose instability, at least during short periods, is involved in the mesangial matrix overproduction observed in diabetes. Copyright (c) 2008 S. Karger AG, Baselen
dc.description.affiliationUniversidade Federal de São Paulo, Div Renal, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Renal, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent222-229
dc.identifierhttp://dx.doi.org/10.1159/000156716
dc.identifier.citationAmerican Journal of Nephrology. Basel: Karger, v. 29, n. 3, p. 222-229, 2009.
dc.identifier.doi10.1159/000156716
dc.identifier.issn0250-8095
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31215
dc.identifier.wosWOS:000261132000009
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofAmerican Journal of Nephrology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.karger.com/Services/RightsPermissions
dc.subjectGlucose deprivationen
dc.subjectGlucose instabilityen
dc.subjectGlucose transportersen
dc.subjectMesangial cellen
dc.subjectDiabetic nephropathyen
dc.titleEffects of Glucose Deprivation or Glucose Instability on Mesangial Cells in Cultureen
dc.typeinfo:eu-repo/semantics/article
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