Biofilm production and evaluation of antifungal susceptibility amongst clinical Candida spp. isolates, including strains of the Candida parapsilosis complex

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dc.contributor.authorMelo, Analy Salles de Azevedo [UNIFESP]
dc.contributor.authorBizerra, Fernando Cesar [UNIFESP]
dc.contributor.authorFreymüller-Haapalainen, Edna [UNIFESP]
dc.contributor.authorArthington-Skaggs, Beth A.
dc.contributor.authorColombo, Arnaldo Lopes [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionCtr Dis Control & Prevent
dc.date.accessioned2016-01-24T14:06:20Z
dc.date.available2016-01-24T14:06:20Z
dc.date.issued2011-04-01
dc.description.abstractCandida cells can form biofilms that frequently are sources of infections and are less susceptible to antifungal drugs. Some authors have reported that Candida orthopsilosis and Candida metapsilosis isolates are not able to produce biofilms in vitro and there are no studies available on biofilm susceptibility for these species to antifungals. the aims of this study were to (i) quantify Candida spp. biofilms in vitro, and (ii) test the in vitro susceptibilities of Candida spp. biofilms to fluconazole (FLC) and amphotericin B (AMB). Isolates studied included four Candida albicans, six C. tropicalis, seven C. parapsilosis, eight C. orthopsilosis, and five C. metapsilosis. We compared two different methods to evaluate biofilm production, i. e., crystal violet (CV) staining and XTT-reduction assays (XTT). Scanning electron microscopy (SEM) was used to observe high, medium and low biofilm producing isolates screened by these two methods. To determine the minimum biofilm eradication concentration (MBEC) for FLC and AMB, XTT-reduction assay was used to measure cell metabolic activity. Biofilm quantification by CV and XTT showed that C. tropicalis isolates were the highest biofilm producer, followed by C. albicans, C. parapsilosis, C. orthopsilosis and C. metapsilosis. Examination of SEM images revealed that the extent of biofilms formed by high, medium, and low producers was highly correlated to the results generated by CV assay. Biofilm of all the isolates evaluated were resistant to FLC (MBEC(80) >= 256 ug/ml) but, in general, susceptible to AMB, except for six C. parapsilosis strains (MBEC (80) >= 8 ug/ml).en
dc.description.affiliationUniversidade Federal de São Paulo, Disciplina Infectol, Lab Especial Micol, Div Infect Dis, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Ctr Electron Microscopy, BR-04023062 São Paulo, Brazil
dc.description.affiliationCtr Dis Control & Prevent, Mycot Dis Branch, Atlanta, GA USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Disciplina Infectol, Lab Especial Micol, Div Infect Dis, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Ctr Electron Microscopy, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCDC (Centers for Disease Control and Prevention, USA)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2005/50778-1
dc.description.sponsorshipIDCNPq: 304802/2007-7
dc.format.extent253-262
dc.identifierhttp://dx.doi.org/10.3109/13693786.2010.530032
dc.identifier.citationMedical Mycology. London: Informa Healthcare, v. 49, n. 3, p. 253-262, 2011.
dc.identifier.doi10.3109/13693786.2010.530032
dc.identifier.issn1369-3786
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33581
dc.identifier.wosWOS:000288323400004
dc.language.isoeng
dc.publisherInforma Healthcare
dc.relation.ispartofMedical Mycology
dc.rightsAcesso aberto
dc.rights.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.subjectCandida spp.en
dc.subjectbiofim production and susceptibilityen
dc.subjectCandida orthopsilosisen
dc.subjectCandida metapsilosisen
dc.titleBiofilm production and evaluation of antifungal susceptibility amongst clinical Candida spp. isolates, including strains of the Candida parapsilosis complexen
dc.typeArtigo
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