Characterization of Schistosoma mansoni ATPDase2 gene, a novel apyrase family member

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dc.contributor.authorLevano-Garcia, Julio
dc.contributor.authorMortara, Renato A.
dc.contributor.authorVerjovski-Almeida, Sergio
dc.contributor.authorDeMarco, Ricardo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:41:49Z
dc.date.available2016-01-24T12:41:49Z
dc.date.issued2007-01-12
dc.description.abstractSchistosoma mansoni is a major causative agent of schistosomiasis, which constitutes a severe health problem in developing countries. We have previously described the SmATPDase1 gene, encoding a protein from the external surface of the parasites. in this work, we describe the cloning and characterization of SmATPDase2, a novel C1339-like ATP diphosphohydrolase gene in S. mansoni. in silico analysis of the protein encoded by SmATPDase2 predicts a single N-terminal transmembrane domain similar to that described for secreted human apyrase isoforms. Immuno-colocalization experiments detected both SmATPDase proteins at the S. mansoni adult worm tegument basal and apical membranes, but only SmATPDase2 in the tegument syncytium. SmATPDase2 but not SmATPDase1 protein was detected by Western blot in culture medium supernatants following incubation of adult worms in vitro, indicating that SmATPDase2 was secreted by the parasite to the medium. Taken together these data suggest a non-redundant role for SmATPDase2 in the parasite-host interplay. (c) 2006 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Bioquim, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent384-389
dc.identifierhttp://dx.doi.org/10.1016/j.bbrc.2006.11.023
dc.identifier.citationBiochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 352, n. 2, p. 384-389, 2007.
dc.identifier.doi10.1016/j.bbrc.2006.11.023
dc.identifier.issn0006-291X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29455
dc.identifier.wosWOS:000243196300017
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectSchistosoma mansonien
dc.subjectATP-diphosphohydrolase isoformsen
dc.subjectapyraseen
dc.subjectcloningen
dc.subjecttegumenten
dc.titleCharacterization of Schistosoma mansoni ATPDase2 gene, a novel apyrase family memberen
dc.typeinfo:eu-repo/semantics/article
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