Aerobic exercise training improves oxidative stress and ubiquitin proteasome system activity in heart of spontaneously hypertensive rats

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dc.contributor.authorAndrade, Luiz Henrique Soares de [UNIFESP]
dc.contributor.authorMoraes, Wilson Max Almeida Monteiro de [UNIFESP]
dc.contributor.authorMatsuo Junior, Eduardo Hiroshi [UNIFESP]
dc.contributor.authorMoura, Elizabeth de Orleans Carvalho de [UNIFESP]
dc.contributor.authorAntunes, Hanna Karen Moreira [UNIFESP]
dc.contributor.authorMontemor, Jairo [UNIFESP]
dc.contributor.authorAntonio, Ednei Luiz [UNIFESP]
dc.contributor.authorBocalini, Danilo Sales [UNIFESP]
dc.contributor.authorSerra, Andrey Jorge [UNIFESP]
dc.contributor.authorTucci, Paulo José Ferreira [UNIFESP]
dc.contributor.authorBrum, Patricia Chakur
dc.contributor.authorMedeiros, Alessandra [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Sao Judas Tadeu
dc.contributor.institutionUniv Nove Julho
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:40:18Z
dc.date.available2016-01-24T14:40:18Z
dc.date.issued2015-04-01
dc.description.abstractThe activity of the ubiquitin proteasome system (UPS) and the level of oxidative stress contribute to the transition from compensated cardiac hypertrophy to heart failure in hypertension. Moreover, aerobic exercise training (AET) is an important therapy for the treatment of hypertension, but its effects on the UPS are not completely known. the aim of this study was to evaluate the effect of AET on UPS's activity and oxidative stress level in heart of spontaneously hypertensive rats (SHR). A total of 53 Wi-star and SHR rats were randomly divided into sedentary and trained groups. the AET protocol was 59/week in treadmill for 13 weeks. Exercise tolerance test, non-invasive blood pressure measurement, echocardiographic analyses, and left ventricle hemodynamics were performed during experimental period. the expression of ubiquitinated proteins, 4-hydroxynonenal (4-HNE), Akt, phospho-Akt(ser473), GSK3 beta, and phospho-GSK3 beta(ser9) were analyzed by western blotting. the evaluation of lipid hydroperoxide concentration was performed using the xylenol orange method, and the proteasomal chymotrypsin-like activity was measured by fluorimetric assay. Sedentary hypertensive group presented cardiac hypertrophy, unaltered expression of total Akt, phospho-Akt, total GSK3 beta and phospho-GSK3 beta, UPS hyperactivity, increased lipid hydroperoxidation as well as elevated expression of 4-HNE but normal cardiac function. in contrast, AET significantly increased exercise tolerance, decreased resting systolic blood pressure and heart rate in hypertensive animals. in addition, the AET increased phospho-Akt expression, decreased phospho-GSK3 beta, and did not alter the expression of total Akt, total GSK3 beta, and ubiquitinated proteins, however, significantly attenuated 4-HNE levels, lipid hydroperoxidation, and UPS's activity toward normotensive group levels. Our results provide evidence for the main effect of AET on attenuating cardiac ubiquitin proteasome hyperactivity and oxidative stress in SHR rats.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biociencias, Silva Jardim, BR-11015020 Santos, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Cardiophysiol & Pathophysiol Lab, São Paulo, Brazil
dc.description.affiliationUniv Sao Judas Tadeu, Dept Postgrad Phys Educ, São Paulo, Brazil
dc.description.affiliationUniv Nove Julho, Postgrad Program Biophoton Appl Hlth Sci, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Phys Educ & Sport, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biociencias, Silva Jardim, BR-11015020 Santos, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Cardiophysiol & Pathophysiol Lab, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDCNPq: 474085/2011-2
dc.format.extent193-202
dc.identifierhttps://dx.doi.org/10.1007/s11010-015-2326-1
dc.identifier.citationMolecular and Cellular Biochemistry. Dordrecht: Springer, v. 402, n. 1-2, p. 193-202, 2015.
dc.identifier.doi10.1007/s11010-015-2326-1
dc.identifier.issn0300-8177
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/38937
dc.identifier.wosWOS:000352849800019
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofMolecular and Cellular Biochemistry
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectHypertensionen
dc.subjectAerobic exercise trainingen
dc.subjectCardiac remodelingen
dc.subjectUbiquitin proteasome systemen
dc.subjectOxidative stressen
dc.titleAerobic exercise training improves oxidative stress and ubiquitin proteasome system activity in heart of spontaneously hypertensive ratsen
dc.typeinfo:eu-repo/semantics/article
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