Acquisition of Serum Antibodies Reactive With Enterohemorrhagic Escherichia coli Virulence-Associated Factors by Healthy Brazilian Children and Adults

Página do item simplificado
dc.contributor.authorPalmeira, Patricia
dc.contributor.authorCarbonare, Solange B.
dc.contributor.authorGuth, Beatriz E. C. [UNIFESP]
dc.contributor.authorCarbonare, Cristiane B.
dc.contributor.authorPontes, Gerlandia N.
dc.contributor.authorTino-De-Franco, Milene
dc.contributor.authorZapata-Quintanilla, Lucy B.
dc.contributor.authorCarneiro-Sampaio, Magda
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionButantan Inst
dc.date.accessioned2016-01-24T13:58:59Z
dc.date.available2016-01-24T13:58:59Z
dc.date.issued2009-12-01
dc.description.abstractBackground: Patients with hemorrhagic colitis or hemolytic uremic syndrome due to enterohemorrhagic Escherichia coli (EHEC) develop serum IgM and IgG response to lipopolysaccharide (LPS) and to virulence factors such as intimin. the small numbers of cases of diarrhea associated with EHEC strains in Brazil suggests a pre-existing immunity probably due to previous contact with diarrheagenic E. coli. Our aim was to evaluate the development of the serum antibody repertoire to EHEC virulence factors in Brazilian children and adults.Methods: Serum IgM and IgG antibodies were determined by enzyme-linked immunosorbent assay with LPS O111, LPS O26, and LPS O157 in 101 children between 2 months and 10 years of age and in 100 adult sera, by immunoblotting with protein membrane extracts and purified beta intimin; the ability of adult sera to neutralize Shiga toxin2 was also investigated.Results: Children older than 24 months had IgM concentrations reactive with the 3 LPS equivalent to those seen in the adult group, and significantly higher than the group of younger children (P < 0.05). Anti-O26 and anti-O157 LPS IgG concentrations were equivalent between the 2 groups of children and were significantly different from the adult group (P < 0.05). the anti-O111 LPS IgG levels in older children were intermediate between the younger group, and adults (P < 0.05). Immunoblotting revealed strong protein reactivity, including the conserved and variable regions of beta intimin and more than 50% of the adult samples neutralized Shiga toxin 2.Conclusions: Our results demonstrate an increasing anti-LPS and antiprotein antibody response with age, which could provide protection against EHEC infections.en
dc.description.affiliationUniv São Paulo, Sch Med, Dept Pediat, BR-05403000 São Paulo, Brazil
dc.description.affiliationButantan Inst, Immunogenet Lab, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 02/03596-7
dc.description.sponsorshipIDFAPESP: 03/13250-3
dc.format.extent1089-1094
dc.identifierhttp://dx.doi.org/10.1097/INF.0b013e3181aa6b2d
dc.identifier.citationPediatric Infectious Disease Journal. Philadelphia: Lippincott Williams & Wilkins, v. 28, n. 12, p. 1089-1094, 2009.
dc.identifier.doi10.1097/INF.0b013e3181aa6b2d
dc.identifier.issn0891-3668
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32002
dc.identifier.wosWOS:000272225200012
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofPediatric Infectious Disease Journal
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectenterohemorrhagic Escherichia colien
dc.subjectlipopolysaccharideen
dc.subjectStx2en
dc.subjectintiminen
dc.subjectantibody responseen
dc.subjectIgG antibodiesen
dc.subjectIgM antibodiesen
dc.titleAcquisition of Serum Antibodies Reactive With Enterohemorrhagic Escherichia coli Virulence-Associated Factors by Healthy Brazilian Children and Adultsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos