17 beta-estradiol induces the translocation of the estrogen receptors ESR1 and ESR2 to the cell membrane, MAPK3/1 phosphorylation and proliferation of cultured immature rat Sertoli cells

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dc.contributor.authorLucas, Thais Fabiana Gameiro [UNIFESP]
dc.contributor.authorSiu, Erica Rosanna [UNIFESP]
dc.contributor.authorEsteves, Carlos A. [UNIFESP]
dc.contributor.authorMonteiro, Hugo Pequeno [UNIFESP]
dc.contributor.authorOliveira, Cleida Aparecida de
dc.contributor.authorPorto, Catarina Segreti [UNIFESP]
dc.contributor.authorLazari, Maria de Fatima Magalhaes [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.date.accessioned2016-01-24T13:49:18Z
dc.date.available2016-01-24T13:49:18Z
dc.date.issued2008-01-01
dc.description.abstractThe aim of the present study was to determine the mechanisms involved in estrogen actions in cultured rat Sertoli cells. RT-PCR detected transcripts for the estrogen receptors ESR1 and ESR2 in cultured immature Sertoli cells and in the testis of 15-, 28-, and 120-day-old rats. the expression of ESR1 and ESR2 was confirmed in Sertoli cells by immunofluorescence and Western blot. Immunohistochemistry with cryosections of testes from immature and adult rats revealed that ESR1 is present in Sertoli, Leydig, and some peritubular myoid cells, and ESR2 is present in multiple cell types, including germ cells. Treatment of Sertoli cells with 17beta-estradiol (E-2) induced a translocation of ESR1 and ESR2 to the plasma membrane and a, concomitant phosphorylation of MAPK3/1. Both effects reached a maximum after 10 min and were blocked by PP2, an inhibitor of the SRC family of protein tyrosine kinases, and by the antiestrogen ICI 182,780 (10). MAPK3/1 phosphorylation was also decreased in the presence of AG 1478, an inhibitor of the epidermal growth factor receptor (EGFR) kinase, and in the presence of MAP2K1/2 inhibitor UO126. Treatment with 2 for 24 h increased the incorporation of [methyl-H-3]thymidine, which was blocked by ICI. These results indicate that E2 activates an SRC-mediated translocation of estrogen receptors to the plasma membrane, which results in the activation of EGFR and the mitogen-activated protein kinase signaling pathway. in addition, activation of ESR1 and/or ESR2 by E 2 is involved in proliferation of immature Sertoli cells. the estrogen actions in Sertoli cells might be a key step mediating cellular events important for spermatogenesis and fertility.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol,INFAR, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Dept Morphol, BR-31270901 Belo Horizonte, MG, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol,INFAR, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt
dc.description.sponsorshipIDFAPESP: 2004/01152-0pt
dc.format.extent101-114
dc.identifierhttps://dx.doi.org/10.1095/biolreprod.107.063909
dc.identifier.citationBiology of Reproduction. Madison: Soc Study Reproduction, v. 78, n. 1, p. 101-114, 2008.
dc.identifier.doi10.1095/biolreprod.107.063909
dc.identifier.issn0006-3363
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/30227
dc.identifier.wosWOS:000251916000012
dc.language.isoeng
dc.publisherSoc Study Reproduction
dc.relation.ispartofBiology of Reproduction
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectEstradiolen
dc.subjectEstradiol receptoren
dc.subjectKinasesen
dc.subjectMechanisms of hormone actionen
dc.subjectSertoli cellsen
dc.title17 beta-estradiol induces the translocation of the estrogen receptors ESR1 and ESR2 to the cell membrane, MAPK3/1 phosphorylation and proliferation of cultured immature rat Sertoli cellsen
dc.typeinfo:eu-repo/semantics/article
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