Autoantibodies specificity in acute anterior uveitis according to the presence of the HLA-B27 allele
| dc.contributor.author | Trevisani, VFM | |
| dc.contributor.author | Mattos, KTF | |
| dc.contributor.author | Esteves, R. F. | |
| dc.contributor.author | Olialves, SMR | |
| dc.contributor.author | Andrade, LEC | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.date.accessioned | 2016-01-24T12:31:15Z | |
| dc.date.available | 2016-01-24T12:31:15Z | |
| dc.date.issued | 2001-01-01 | |
| dc.description.abstract | Purpose: To study HLA-B27circle plus and HLA-B27O patients with AAU with respect to the frequency and specificity of organ-specific and non-organ-specific autoantibodies. Methods: Fifty-seven consecutive patients with AAU were subjected to ophthalmologic and rheumatologic evaluation as well as to autoantibody determination: antinuclear antibodies (ANA), rheumatoid factor (RF), anticardiolipin, anti-smooth muscle, and anti-parietal cell antibodies, and immunoblot for antibodies to HeLa cells and to bovine iris extract. HLA-B27 was determined by a microlymphocytotoxicity assay. Statistical analysis employed chi-square test, Fisher's exact test, and McNemar test. Results: Thirty-four patients had the HLA-B27 allele (B27circle plus/AAU) and 23 did not (B27O/AAU). ANA, RF, anticardiolipin, and anti-parietal cell antibodies appeared in low frequency. No patient presented anti-smooth muscle antibody. Immunoblot showed a high frequency of antibodies to HeLa cell proteins in B27O/AAU patients with predominant reactivity at 46 kDa and 56kDa. in contrast, sera from B27circle plus/AAU patients reacted poorly against HeLa cell antigens. Immunoblot with bovine iris extract showed a significant frequency of antibodies in both groups, with a predominant response to antigens with an estimated mobility of 35, 52, and 54 kDa. Conclusion: Antibodies specific to iris antigens were equally frequent in both acute uveitis groups, whereas non-organ-specific autoantibodies, especially those to HeLa cell proteins, were far less frequent in B27circle plus/AAU than in B27O/AAU patients. | en |
| dc.description.affiliation | Universidade Federal de São Paulo, Div Rheumatol, São Paulo, Brazil | |
| dc.description.affiliation | Universidade Federal de São Paulo, Div Ophthalmol, São Paulo, Brazil | |
| dc.description.affiliationUnifesp | Universidade Federal de São Paulo, Div Rheumatol, São Paulo, Brazil | |
| dc.description.affiliationUnifesp | Universidade Federal de São Paulo, Div Ophthalmol, São Paulo, Brazil | |
| dc.description.source | Web of Science | |
| dc.format.extent | 231-242 | |
| dc.identifier | http://dx.doi.org/10.1076/ocii.9.4.231.3952 | |
| dc.identifier.citation | Ocular Immunology and Inflammation. Lisse: Swets Zeitlinger Publishers, v. 9, n. 4, p. 231-242, 2001. | |
| dc.identifier.doi | 10.1076/ocii.9.4.231.3952 | |
| dc.identifier.issn | 0927-3948 | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/26455 | |
| dc.identifier.wos | WOS:000174654800002 | |
| dc.language.iso | eng | |
| dc.publisher | Swets Zeitlinger Publishers | |
| dc.relation.ispartof | Ocular Immunology and Inflammation | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.subject | autoantibodies | en |
| dc.subject | anterior uveitis | en |
| dc.subject | HLA-B27 | en |
| dc.title | Autoantibodies specificity in acute anterior uveitis according to the presence of the HLA-B27 allele | en |
| dc.type | info:eu-repo/semantics/article |