Characterization of a 21 kDa protein from Trypanosoma cruzi associated with mammalian cell invasion

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dc.contributor.authorSilva, Claudio V. da [UNIFESP]
dc.contributor.authorKawashita, Silvia Y. [UNIFESP]
dc.contributor.authorProbst, Christian M.
dc.contributor.authorDallagiovanna, Bruno
dc.contributor.authorCruz, Mario C. [UNIFESP]
dc.contributor.authorSilva, Erika A. da [UNIFESP]
dc.contributor.authorSouto-Padron, Thais C. B. S.
dc.contributor.authorKrieger, Marco A.
dc.contributor.authorGoldenberg, Samuel
dc.contributor.authorBriones, Marcelo R. S. [UNIFESP]
dc.contributor.authorAndrews, Norma W.
dc.contributor.authorMortara, Renato A. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFiocruz MS
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionYale Univ
dc.date.accessioned2016-01-24T13:52:24Z
dc.date.available2016-01-24T13:52:24Z
dc.date.issued2009-04-01
dc.description.abstractTrypanosoma cruzi genomic database was screened for hypothetical proteins that showed high probability of being secreted or membrane anchored and thus, likely involved in host-cell invasion. A sequence that codes for a 21 kDa protein that showed high probability of being secreted was selected. After cloning this protein sequence, the results showed that it was a ubiquitous protein and secreted by extracellular amastigotes. the recombinant form (P21-His(6)) adhered to HeLa cells in a dose-dependent manner. Pretreatment of host cells with P21-His(6) inhibited cell invasion by extracellular amastigotes from G and CL strains. On the other hand, when the protein was added to host cells at the same time as amastigotes, an increase in cell invasion was observed. Host-cell pretreatment with P21-His(6) augmented invasion by metacyclic trypomastigotes. Moreover, polyclonal antibody anti-P21 inhibited invasion only by extracellular amastigotes and metacyclic trypomastigotes from G strain. These results suggested that P21 might be involved in T. cruzi cell invasion. We hypothesize that P21 could be secreted in the juxtaposition parasite-host cell and triggers signaling events yet unknown that lead to parasite internalization. (C) 2009 Elsevier Masson SAS. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationFiocruz MS, Inst Biol Mol Parana, Curitiba, Parana, Brazil
dc.description.affiliationFiocruz MS, ICC, Curitiba, Parana, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Dept Microbiol, BR-21941 Rio de Janeiro, Brazil
dc.description.affiliationYale Univ, Sch Med, Sect Microbial Pathogenesis, New Haven, CT USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 03/12962-0
dc.format.extent563-570
dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2009.03.007
dc.identifier.citationMicrobes and Infection. Amsterdam: Elsevier B.V., v. 11, n. 5, p. 563-570, 2009.
dc.identifier.doi10.1016/j.micinf.2009.03.007
dc.identifier.issn1286-4579
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31420
dc.identifier.wosWOS:000267839100004
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMicrobes and Infection
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectTrypanosoma cruzien
dc.subjectCell invasionen
dc.subjectAmastigoteen
dc.subjectMetacyclic trypomastigoteen
dc.titleCharacterization of a 21 kDa protein from Trypanosoma cruzi associated with mammalian cell invasionen
dc.typeinfo:eu-repo/semantics/article
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