Comparative study of the expression of cellular cycle proteins in cervical intraepithelial lesions

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dc.contributor.authorQueiroz, Conceicao
dc.contributor.authorSilva, Tania Correia
dc.contributor.authorAlves, Venancio A. F. [UNIFESP]
dc.contributor.authorVilla, Luisa L.
dc.contributor.authorCosta, Maria Cecilia
dc.contributor.authorTravassos, Ana Gabriela
dc.contributor.authorAraujo, Jose Bouzas
dc.contributor.authorStudart, Eduardo
dc.contributor.authorCheto, Tatiana
dc.contributor.authorFreitas, Luiz Antonio R. de
dc.contributor.institutionUniversidade Federal da Bahia (UFBA)
dc.contributor.institutionFiocruz MS
dc.contributor.institutionLudwig Inst Canc Res
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:38:17Z
dc.date.available2016-01-24T12:38:17Z
dc.date.issued2006-01-01
dc.description.abstractInteraction of human papilloma virus oncoproteins E6 and E7 with cell cycle proteins leads to disturbances of the cell cycle mechanism and subsequent alteration in the expression of some proteins, such as p16(INK4a), cyclin DI, p53 and KI67. in this study, we compared alterations in the expression of these proteins during several stages of intra-epitelial cervical carcinogenesis. Accordingly, an immunohistochemical study was performed on 50 cervical biopsies, including negative cases and intraepithelial neoplasias. the expression patterns of these markers were correlated with the histopathological diagnosis and infection with HPV. the p16(INK4a), followed by Ki67, showed better correlation with cancer progression than p53 and cyclin DI, which recommends their use in the evaluation of cervical carcinogenesis. These monoclonal antibodies can be applied to cervical biopsy specimens to identify lesions transformed by oncogenic HPV, separating CIN 1 (p16(INK4a) positive) and identifying high-grade lesions by an increase in the cellular proliferation index (Ki67). in this way, we propose immunomarkers that can be applied in clinical practice to separate patients who need a conservative therapeutic approach from those who require a more aggressive treatment. (c) 2006 Elsevier GmbH. All rights reserved.en
dc.description.affiliationUniv Fed Bahia, Sch Med, Dept Pathol, Dept Gynecol & Obstet, Salvador, BA, Brazil
dc.description.affiliationUniv Fed Bahia, Sch Math & Stat, Salvador, BA, Brazil
dc.description.affiliationFiocruz MS, Oswaldo Cruz Fdn Goncali Moniz Res Ctr, Salvador, BA, Brazil
dc.description.affiliationLudwig Inst Canc Res, Dept Virol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Adolfo Lutz Inst, Dept Pathol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Adolfo Lutz Inst, EPM, Dept Pathol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent731-737
dc.identifierhttp://dx.doi.org/10.1016/j.prp.2006.07.003
dc.identifier.citationPathology Research and Practice. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 202, n. 10, p. 731-737, 2006.
dc.identifier.doi10.1016/j.prp.2006.07.003
dc.identifier.issn0344-0338
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28653
dc.identifier.wosWOS:000241498800005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPathology Research and Practice
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectcervical neoplasiaen
dc.subjectp16(INK4a)en
dc.subjectcyclin D1en
dc.subjectKi67en
dc.subjectp53en
dc.titleComparative study of the expression of cellular cycle proteins in cervical intraepithelial lesionsen
dc.typeinfo:eu-repo/semantics/article
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