The Role of Host Factors and Bacterial Virulence Genes in the Development of Pyelonephritis Caused by Escherichia coli in Renal Transplant Recipients

Página do item simplificado
dc.contributor.authorSiliano, Priscila Reina [UNIFESP]
dc.contributor.authorRocha, Lillian Andrade [UNIFESP]
dc.contributor.authorPestana, Jose Osmar Medina [UNIFESP]
dc.contributor.authorHeilberg, Ita Pfeferman [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:05Z
dc.date.available2016-01-24T14:05:05Z
dc.date.issued2010-07-01
dc.description.abstractBackground and objectives: the aim of this study was to determine the role of host factors and bacterial virulence genes in the development of pyelonephritis caused by Escherichia coli in renal transplant (Tx) recipients.Design, setting, participants, & measurements: A total of 328 E. coli isolates from cases of cystitis (Cys; n = 239) or pyelonephritis (PN; n = 89), with 169 from renal Tx recipients, were subjected to molecular analyses to identify P-fimbria subunits (PapC, PapG II, and PapGIII), G- and M-fimbriae, and aerobactin. the presence of antibiotic resistance was also determined. Parameters such as gender, age, immunosuppression regimens, causes of ESRD, kidney donor, intraoperative anastomosis, use of double J stent, trimethoprim/sulfamethoxazole (TMP/SMZ) prophylaxis, and time after Tx were evaluated.Results: A multivariate analysis showed a significant association between PN and renal Tx. in renal Tx recipients, the risk of occurrence of PN was significantly higher among males and for those no longer receiving TMP/SMZ prophylaxis. E. coli strains isolated from PN presented a lower prevalence of papGIII and lower rates of resistance to pipemidic acid. Although papGII was more prevalent in PN than in Cys, it was not independently associated with PN.Conclusions: These findings suggested that renal Tx increases the risk for PN, and the male sex represented a host factor independently associated with risk, whereas the prophylaxis with TMP/SMZ was protective. the lack of papGIII and low resistance to first-generation quinolones were bacterial-independent risk factors for PN in Tx. Clin J Am Soc Nephrol 5: 1290-1297, 2010. doi: 10.2215/CJN.06740909en
dc.description.affiliationUniversidade Federal de São Paulo, Div Nephrol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Nephrol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundacao Oswaldo Ramos
dc.format.extent1290-1297
dc.identifierhttp://dx.doi.org/10.2215/CJN.06740909
dc.identifier.citationClinical Journal of the American Society of Nephrology. Washington: Amer Soc Nephrology, v. 5, n. 7, p. 1290-1297, 2010.
dc.identifier.doi10.2215/CJN.06740909
dc.identifier.issn1555-9041
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32659
dc.identifier.wosWOS:000279752700020
dc.language.isoeng
dc.publisherAmer Soc Nephrology
dc.relation.ispartofClinical Journal of the American Society of Nephrology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleThe Role of Host Factors and Bacterial Virulence Genes in the Development of Pyelonephritis Caused by Escherichia coli in Renal Transplant Recipientsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos