Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis

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dc.contributor.authorSuffys, P.
dc.contributor.authorPalomino, Juan Carlos
dc.contributor.authorLeao, Sylvia Cardoso [UNIFESP]
dc.contributor.authorEspitia, C.
dc.contributor.authorCataldi, A.
dc.contributor.authorAlito, A.
dc.contributor.authorVelasco, M.
dc.contributor.authorRobledo, J.
dc.contributor.authorFernandez, J.
dc.contributor.authorRosa, P. D.
dc.contributor.authorRomano, M. I.
dc.contributor.institutionCICV
dc.contributor.institutionFiocruz MS
dc.contributor.institutionUniv Peruana Cayetano Heredia
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Nacl Autonoma Mexico
dc.contributor.institutionInst Salud Publ Chile
dc.contributor.institutionCorporac Invest Biol
dc.date.accessioned2018-06-15T17:49:48Z
dc.date.available2018-06-15T17:49:48Z
dc.date.issued2000-02-01
dc.description.abstractThe development of nucleic acid-based technologies has improved the sensitivity, specificity and speed of detection of Mycobacterium tuberculosis in clinical samples. Both commercially available and 'in-house' polymerase chain reaction (PCR) systems are in use, and a significant number of reports compare such systems with more traditional diagnostic tools for tuberculosis. Few studies, however, have focused on the reproducibility of the results when submitting a sample batch to PCR in different laboratories, especially in developing countries. Consequently, PCR results obtained from six laboratories in six different Latin American countries for samples reconstituted with defined amounts of M. tuberculosis cells were evaluated. Each laboratory used specific conditions of sample processing, nucleic acid amplification and amplicon detection. Analysis of results allowed large differences in sensitivity and specificity to be observed. We conclude that in its present setting, inhouse PCR cannot be used as a single diagnostic tool for tuberculosis, and that special care needs to be taken upon interpretation of results by inclusion of a proper number of positive and negative controls.en
dc.description.affiliationCICV, Inst Biotecnol, INTA, RA-1708 Buenos Aires, DF, Argentina
dc.description.affiliationFiocruz MS, Dept Bioquim & Biol Mol, Lab Diagnost Doencas Infecciosas, BR-21045900 Rio De Janeiro, Brazil
dc.description.affiliationUniv Peruana Cayetano Heredia, Lima, Peru
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUniv Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City, DF, Mexico
dc.description.affiliationInst Salud Publ Chile, Secc Micobacterias, Santiago, Chile
dc.description.affiliationInst Salud Publ Chile, Unidad Biol Mol, Santiago, Chile
dc.description.affiliationCorporac Invest Biol, Medellin, Colombia
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent179-183
dc.identifierhttp://www.ingentaconnect.com/contentone/iuatld/ijtld/2000/00000004/00000002/art00015#aff_4
dc.identifier.citationInternational Journal Of Tuberculosis And Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 4, n. 2, p. 179-183, 2000.
dc.identifier.issn1027-3719
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/44117
dc.identifier.wosWOS:000085339300015
dc.language.isoeng
dc.publisherInt Union Against Tuberculosis Lung Disease (i U A T L D)
dc.relation.ispartofInternational Journal Of Tuberculosis And Lung Disease
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjecttuberculosisen
dc.subjectPCRen
dc.subjectMycobacterium tuberculosisen
dc.titleEvaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosisen
dc.typeinfo:eu-repo/semantics/article
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