Distribution of I-125-labeled crotamine in mice tissues

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dc.contributor.authorBoni-Mitake, M.
dc.contributor.authorCosta, H.
dc.contributor.authorVassilleff, S.
dc.contributor.authorRogero, J. R.
dc.contributor.institutionServ Radioprotecao
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionIPEN
dc.date.accessioned2016-01-24T12:41:31Z
dc.date.available2016-01-24T12:41:31Z
dc.date.issued2006-10-01
dc.description.abstractCrotamine is a strong basic polypeptide from Crotalus durissus terrificus (Cdt) venom composed of 42 amino acid residues tightly bound by three disulfide bonds. It causes skeletal muscle spasms leading to spastic paralysis of hind limbs in mice. the objective of this paper was to study the distribution of crotamine injected intraperitoneally (ip) in mice. Crotamine was purified from Cdt venom by gel filtration, followed by ion exchange chromatography, using a fast-performance liquid chromatography (FPLC) system. Purified crotamine was irradiated at 2 kGy in order to detoxify. Both native and irradiated proteins were labeled with 125, using chloramine T method, and separated by get filtration. Male Swiss mice were injected ip with 0.1 mL (2 x 10(6) cpm/mouse) of I-125 native or irradiated crotamine. At various time intervals, the animals were killed by ether inhalation and blood, spleen, liver, kidneys, brain, lungs, heart, and skeletal muscle were collected in order to determine the radioactivity content. the highest levels of radioactivity were found in the kidneys and the liver, and the lowest in the brain. (c) 2006 Elsevier B.V. All rights reserved.en
dc.description.affiliationServ Radioprotecao, BR-05508000 São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ginecol, BR-04039032 São Paulo, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Ctr Assisten Toxicol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationIPEN, Ctr Biol Mol, CNEN SP, BR-05508000 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, EPM, Dept Ginecol, BR-04039032 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent550-555
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2006.07.005
dc.identifier.citationToxicon. Oxford: Pergamon-Elsevier B.V., v. 48, n. 5, p. 550-555, 2006.
dc.identifier.doi10.1016/j.toxicon.2006.07.005
dc.identifier.issn0041-0101
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29194
dc.identifier.wosWOS:000241328700009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofToxicon
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectcrotamineen
dc.subjecttoxinen
dc.subjectbiodistributionen
dc.subjectCrotalusen
dc.subjectvenomen
dc.subjectgamma radiationen
dc.titleDistribution of I-125-labeled crotamine in mice tissuesen
dc.typeinfo:eu-repo/semantics/article
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