Genotoxicity of Endosseous Implants Using Two Cellular Lineages in Vitro

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dc.contributor.authorMatsumoto, Mariza Akemi
dc.contributor.authorNary Filho, Hugo
dc.contributor.authorFerrari, Raquel
dc.contributor.authorFernandes, Kristianne
dc.contributor.authorRenno, Ana Claudia Muniz [UNIFESP]
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.institutionUniv Sagrado Coracao
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Nove de Julho
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:35:17Z
dc.date.available2016-01-24T14:35:17Z
dc.date.issued2014-02-01
dc.description.abstractThe genotoxic potential of corrosion eluates obtained from a single dental implant using murine fibroblasts or osteoblasts cells in vitro by the single-cell gel (comet) assay was examined. A single commercially available dental implant (Biotechnology) was eluted in a solution consisting of equal amounts of acetic acid and sodium chloride (0.1 M) for 1, 3, 7, 14, and 21 days. Murine fibroblast or osteoblast cultures were then exposed to all corrosion eluates obtained from endosseous dental implants for 30 minutes at 37 degrees C. the results suggest that none of the eluates produced genotoxic changes in murine fibroblasts regardless of the length of exposure to the eluate. Similarly, no genotoxicity was found in osteoblasts. the results suggest that the dental implant eluates tested in this study did not induce genetic damage as depicted by the single-cell gel (comet) assay. Because DNA damage is an important event during oncogenesis, this study represents a relevant contribution to estimate the real risks to the cellular system induced by the corrosion products of a dental implant.en
dc.description.affiliationUniv Sagrado Coracao, Dept Oral & Maxillofacial Surg, Usc, SP, Brazil
dc.description.affiliationPrivate Clin, Bauru, SP, Brazil
dc.description.affiliationUniv Nove de Julho, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Santos, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent25-30
dc.identifierhttps://dx.doi.org/10.1563/AAID-JOI-D-10-00112
dc.identifier.citationJournal of Oral Implantology. Lawrence: Allen Press Inc, v. 40, n. 1, p. 25-30, 2014.
dc.identifier.doi10.1563/AAID-JOI-D-10-00112
dc.identifier.issn0160-6972
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/37419
dc.identifier.wosWOS:000333657100006
dc.language.isoeng
dc.publisherAllen Press Inc
dc.relation.ispartofJournal of Oral Implantology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectEndosseous implanten
dc.subjectGenotoxicityen
dc.subjectMouse fibroblast cellsen
dc.subjectOsteoblastsen
dc.titleGenotoxicity of Endosseous Implants Using Two Cellular Lineages in Vitroen
dc.typeinfo:eu-repo/semantics/article
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