Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections

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dc.contributor.authorSampaio, JLM [UNIFESP]
dc.contributor.authorChimara, Erica
dc.contributor.authorFerrazoli, Lucilaine
dc.contributor.authorTelles, Maria Alice da Silva
dc.contributor.authorDel Guercio, VMF
dc.contributor.authorJerico, ZVN
dc.contributor.authorMiyashiro, Kozue [UNIFESP]
dc.contributor.authorFortaleza, CMCB
dc.contributor.authorPadoveze, M. C.
dc.contributor.authorLeao, S. C. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFleury Ctr Med Diagnost
dc.contributor.institutionInst Adolfo Lutz Registro
dc.contributor.institutionInst Vozza Med & Diagnose LTDA
dc.contributor.institutionCtr Vigilancia Epidemiol Prof Alexandre Vranjac
dc.date.accessioned2016-01-24T12:40:56Z
dc.date.available2016-01-24T12:40:56Z
dc.date.issued2006-02-01
dc.description.abstractA cluster of cases of post-augmentation mammaplasty surgical site infections occurred between 2002 and 2004 in Campinas, in the southern region of Brazil. Rapidly growing mycobacteria were isolated from samples from 12 patients. Eleven isolates were identified as Mycobacterium fortuitum and one as Mycobacterium porcinum by PCR-restriction digestion of the hsp65 gene. These 12 isolates, plus six additional M. fortuitum isolates from non-related patients, were typed by pulsed-field gel electrophoresis (PFGE) and three PCR-based techniques: 16S-23S rRNA internal transcribed spacer (ITS) genotyping; randomly amplified polymorphic DNA (RAPD) PCR; and enterobacterial repetitive intergenic consensus (ERIC) PCR. Four novel M. fortuitum allelic variants were identified by restriction analysis of the ITS fragment. One major cluster, comprising six M. fortuitum isolates, and a second cluster of two isolates, were identified by the four methods. RAPD-PCR and ITS genotyping were less discriminative than ERIC-PCR. ERIC-PCR was comparable to PFGE as a valuable complementary tool for investigation of this type of outbreak.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, SP, Brazil
dc.description.affiliationFleury Ctr Med Diagnost, São Paulo, Brazil
dc.description.affiliationInst Adolfo Lutz Registro, São Paulo, Brazil
dc.description.affiliationInst Vozza Med & Diagnose LTDA, Campinas, Brazil
dc.description.affiliationCtr Vigilancia Epidemiol Prof Alexandre Vranjac, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, SP, Brazil
dc.description.provenanceMade available in DSpace on 2016-01-24T12:40:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-02-01. Added 1 bitstream(s) on 2016-01-31T13:33:21Z : No. of bitstreams: 1 WOS000234698700008.pdf: 260855 bytes, checksum: a08dc613692eb2c481d2a997cee6ef07 (MD5)en
dc.description.sourceWeb of Science
dc.format.extent142-149
dc.identifierhttp://dx.doi.org/10.1111/j.1469-0691.2005.01312.x
dc.identifier.citationClinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 12, n. 2, p. 142-149, 2006.
dc.identifier.doi10.1111/j.1469-0691.2005.01312.x
dc.identifier.fileWOS000234698700008.pdf
dc.identifier.issn1198-743X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28709
dc.identifier.wosWOS:000234698700008
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofClinical Microbiology and Infection
dc.rightsAcesso aberto
dc.subjectinfectionsen
dc.subjectmammaplastyen
dc.subjectmolecular epidemiologyen
dc.subjectMycobacterium fortuitumen
dc.subjectplastic surgeryen
dc.subjecttypingen
dc.titleApplication of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infectionsen
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