Nicotinic receptor/ionic channel complex (AChR) in androgen-dependent skeletal muscle cultures

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dc.contributor.authorBielavsky, M.
dc.contributor.authorSouccar, C.
dc.contributor.authorLapa, A. J.
dc.contributor.authorLima-Landman, MTR
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:33:14Z
dc.date.available2016-01-24T12:33:14Z
dc.date.issued2002-02-01
dc.description.abstractThe kinetic properties of the nicotinic receptor/ionic channel complex (AChR) were compared in cell cultures obtained from androgen-dependent skeletal muscles of the perineal complex (P) and from muscles less dependent upon sex hormones (the thigh musculature, T). Because the development of P is delayed compared to other skeletal muscles in the rat, cultures were performed taking into account the age of the donor (4- or 6-day-old rats), and the time interval the cells remained in culture (7 days and 15 days). the ionic channel conductance (gamma) and the mean channel open time (2) were determined with the patch-clamp technique in the cell-attached configuration at room temperature. Cultures from P and T muscles were morphologically identical in size and shape, independent of the animals' age at plating or on the plating time. in all of them, the AChR was spread over the cell membrane. More than one AChR ionic channel conductance was observed in P and T cultures, and the prevalent value of gamma in either culture ranged from 30 pS to 35 pS. in P fibers from 4-day-old rats cultured for 7 days (P 4/7), the distribution of channel open times fitted a double exponential, while in T 4/7 they were fitted with a single exponential. in cultures from P and T muscles obtained from older rats (6 days old) and in those cells remaining in culture for a prolonged time (15 days), the channel open times also fitted a double exponential. Because P and T cultures lack trophic neuronal influences, the difference observed between the tau of P 4/7 and T 4/7 was thought to be the hormone requirement of P muscles to grow and differentiate. Likewise, the difference observed between T 4/7 and T 4/15 may indicate the need for neurotrophic influences to maintain higher T values in older cultures. Since this requirement is not found in cultured fibers, tau would tend to assume slower values approaching those of P without hormone activation.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent595-600
dc.identifierhttp://dx.doi.org/10.1007/s00424-001-0731-7
dc.identifier.citationPflugers Archiv-european Journal of Physiology. New York: Springer-verlag, v. 443, n. 4, p. 595-600, 2002.
dc.identifier.doi10.1007/s00424-001-0731-7
dc.identifier.issn0031-6768
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26745
dc.identifier.wosWOS:000174363000011
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofPflugers Archiv-european Journal of Physiology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectnicotinic receptoren
dc.subjectandrogenen
dc.subjectionic channel kineticsen
dc.subjectcell cultureen
dc.titleNicotinic receptor/ionic channel complex (AChR) in androgen-dependent skeletal muscle culturesen
dc.typeinfo:eu-repo/semantics/article
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