A recombinant cysteine proteinase from Leishmania (Leishmania) chagasi as an antigen for delayed-type hypersensitivity assays and serodiagnosis of canine visceral leishmaniasis

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dc.contributor.authorCosta Pinheiro, Paulo Henrique da
dc.contributor.authorPinheiro, Adriana Nunes
dc.contributor.authorFerreira, Josie Haydée Lima [UNIFESP]
dc.contributor.authorLima Costa, Francisco Assis
dc.contributor.authorKatz, Simone
dc.contributor.authorBarbieri, Clara Lucia [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFac NOVAFAPI
dc.contributor.institutionUniv Fed Piaui
dc.date.accessioned2016-01-24T13:52:33Z
dc.date.available2016-01-24T13:52:33Z
dc.date.issued2009-05-26
dc.description.abstractA recombinant protein, rLdccys1, produced by expression of the gene encoding a 30 kDa cysteine proteinase from Leishmania (Leishmania) chagasi, was used to detect specific antibodies in serum by enzyme-linked immunosorbent assays and to test for reactivity in delayed-type hypersensitivity (DTH) responses of dogs from an endemic region of visceral leishmaniasis (VL), Teresina, Piaui State, Brazil. Amastigote or promastigote extracts were also assayed for comparison. the sensitivity for detection of specific antibodies to L. (L) chagasi using rLdccys1 I, lysates from L. (L.) chagasi promastigotes and amastigotes was 96%, 68%, and 69%, respectively. No cross-reactivity between rLdccys1 and Chagas disease was observed, and little reactivity was found with sera from dogs with babesiosis and ehrlichiosis. Among 106 sera from symptomatic dogs and 22 from non-infected controls, no false negatives and only two false positive sera were found for rLdccys1. in contrast, amastigote lysates yielded 11 false positives and 13 false negatives, whereas the corresponding numbers for promastigote lysates were 17 and 16. DTH responses were determined after intradermal injection of rLdccys1 or amastigote extract and the induration area was measured at 24, 48 and 72 h after injection. All asymptomatic dogs showed a positive intradermal response to rLdccys1 (>10 mm) which peaked at 48 h, whereas no significant reactivity to the recombinant antigen was found in the symptomatic group. Histological analysis of the intradermal induration showed a predominance of necrotic and hemorrhagic areas in sections from asymptomatic dogs injected with L. (L.) chagasi amastigote extract, whereas a typical granulomatous reaction mediated by mononuclear cells was observed in sections from asymptomatic animals injected with rLdccys1. Grouping data from ELISA and DTH assays with rLdccys1 and L. (L) chagasi amastigote extracts showed that humoral and cellular responses were inversely correlated during the development of canine VL. Overall, these findings indicate that L (L) chagasi recombinant cysteine proteinase is potentially useful for diagnosis of canine VL, as well as for the discrimination of clinical and subclinical forms of the disease. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Div Parasitol, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationFac NOVAFAPI, Div Parasitol, Teresina, Piaui, Brazil
dc.description.affiliationFac NOVAFAPI, Div Histol, Teresina, Piaui, Brazil
dc.description.affiliationUniv Fed Piaui, Picos, Piaui, Brazil
dc.description.affiliationUniv Fed Piaui, Dept Vet Clin & Surg, Teresina, Piaui, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Parasitol, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFaculdade NOVAFAPI of Brazil
dc.format.extent32-39
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2009.02.011
dc.identifier.citationVeterinary Parasitology. Amsterdam: Elsevier B.V., v. 162, n. 1-2, p. 32-39, 2009.
dc.identifier.doi10.1016/j.vetpar.2009.02.011
dc.identifier.issn0304-4017
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31527
dc.identifier.wosWOS:000266578200005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofVeterinary Parasitology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectLeishmania (Leishmania) chagasien
dc.subjectCanine visceral leishmaniasisen
dc.subjectSerodiagnosisen
dc.subjectDelayed-type hypersensitivityen
dc.subjectCysteine proteinaseen
dc.titleA recombinant cysteine proteinase from Leishmania (Leishmania) chagasi as an antigen for delayed-type hypersensitivity assays and serodiagnosis of canine visceral leishmaniasisen
dc.typeinfo:eu-repo/semantics/article
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