Characterization and biocompatibility of a fibrous glassy scaffold

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dc.citation.issue4
dc.citation.volume11
dc.contributor.authorGabbai-Armelin, Paulo Roberto
dc.contributor.authorSouza, M. T.
dc.contributor.authorKido, Hueliton Wilian
dc.contributor.authorTim, Carla Roberta
dc.contributor.authorBossini, Paulo Sergio [UNIFESP]
dc.contributor.authorFernandes, Kelly Rossetti [UNIFESP]
dc.contributor.authorMagri, Angela Maria Paiva [UNIFESP]
dc.contributor.authorParizotto, Nivaldo Antonio
dc.contributor.authorFernandes, Kristianne Porta Santos
dc.contributor.authorMesquita-Ferrari, Raquel Agnelli
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorZanotto, Edgar Dutra
dc.contributor.authorPeitl Filho, Oscar [UNIFESP]
dc.contributor.authorRenno, Ana Claudia Muniz [UNIFESP]
dc.coverageHoboken
dc.date.accessioned2020-07-17T14:02:31Z
dc.date.available2020-07-17T14:02:31Z
dc.date.issued2017
dc.description.abstractBioactive glasses (BGs) are known for their ability to bond to living bone and cartilage. In general, they are readily available in powder and monolithic forms, which are not ideal for the optimal filling of bone defects with irregular shapes. In this context, the development of BG-based scaffolds containing flexible fibres is a relevant approach to improve the performance of BGs. This study is aimed at characterizing a new, highly porous, fibrous glassy scaffold and evaluating its in vitro and in vivo biocompatibility. The developed scaffolds were characterized in terms of porosity, mineralization and morphological features. Additionally, fibroblast and osteoblast cells were seeded in contact with extracts of the scaffolds to assess cell proliferation and genotoxicity after 24, 72 and 144 h. Finally, scaffolds were placed subcutaneously in rats for 15, 30 and 60 days. The scaffolds presented interconnected porous structures, and the precursor bioglass could mineralize a hydroxyapatite (HCA) layer in simulated body fluid (SBF) after only 12 h. The biomaterial elicited increased fibroblast and osteoblast cell proliferation, and no DNA damage was observed. The in vivo experiment showed degradation of the biomaterial over time, with soft tissue ingrowth into the degraded area and the presence of multinucleated giant cells around the implant. At day 60, the scaffolds were almost completely degraded and an organized granulation tissue filled the area. The results highlight the potential of this fibrous, glassy material for bone regeneration, due to its bioactive properties, non-cytotoxicity and biocompatibility. Future investigations should focus on translating these findings to orthotopic applications. Copyright (c) 2015 John Wiley & Sons, Ltd.en
dc.description.affiliationFed Univ Sao Carlos UFSCar, Postgrad Programme Biotechnol, Sao Carlos, SP, Brazil
dc.description.affiliationFed Univ Sao Carlos UFSCar, Vitreous Mat Lab LaMaV, Dept Mat Engn, Sao Carlos, SP, Brazil
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Biosci, Ave Ana Costa 95, BR-11050240 Santos, SP, Brazil
dc.description.affiliationFed Univ Sao Carlos UFSCar, Dept Physiotherapy, Sao Carlos, SP, Brazil
dc.description.affiliationNove de Julho Univ UNINOVE, Dept Rehabil Sci & Biophoton Appl Hlth Sci, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Dept Biosci, Ave Ana Costa 95, BR-11050240 Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCentre for Research Technology and Education in Vitreous Materials (CeRTEV)
dc.description.sponsorshipIDCNPq: 303662/2012-3
dc.description.sponsorshipIDCeRTEV: 2013/07793-6
dc.description.sponsorshipIDFAPESP: 2011/22937-9
dc.format.extent1141-1151
dc.identifierhttps://dx.doi.org/10.1002/term.2017
dc.identifier.citationJournal Of Tissue Engineering And Regenerative Medicine. Hoboken, v. 11, n. 4, p. 1141-1151, 2017.
dc.identifier.doi10.1002/term.2017
dc.identifier.issn1932-6254
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54844
dc.identifier.wosWOS:000398815300018
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofJournal Of Tissue Engineering And Regenerative Medicine
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBiocompatibilityen
dc.subjectBiomaterialen
dc.subjectBioactive glassen
dc.subjectFibrous scaffolden
dc.subjectBone repairen
dc.subjectCytotoxicityen
dc.titleCharacterization and biocompatibility of a fibrous glassy scaffolden
dc.typeinfo:eu-repo/semantics/article
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