Paracoccidioides brasiliensis-reactive antibodies in Brazilian blood donors

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dc.contributor.authorBotteon, FAG
dc.contributor.authorCamargo, Zoilo Pires [UNIFESP]
dc.contributor.authorBenard, G.
dc.contributor.authorCoelho, R. F.
dc.contributor.authorChamone, DAF
dc.contributor.authorItano, E. N.
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionHemoctr Reg Londrina
dc.contributor.institutionFdn Pro Sanguel
dc.date.accessioned2016-01-24T12:33:29Z
dc.date.available2016-01-24T12:33:29Z
dc.date.issued2002-08-01
dc.description.abstractIn a survey for primary paracoccidioidomycosis (PCM) infection (and not the clinical disease), two groups of blood donors were analyzed. One study group was drawn from donors living in a rural area where PCM is endemic, and the other group from urban residents of a large city, São Paulo. Anti-Paracoccidioides brasiliensis (Pb) specific antibodies (IgG) in sera were analyzed by ELISA, using crude Pb exoantigens (exoAg) and purified specific Pb 43 kDa glycoprotein (gp43). the results showed that 21% of 700 rural samples and 0.9% of 350 urban samples were positive for exoAg and gp43. To avoid cross-reactions, the sera were adsorbed first with Histoplasma capsulatum antigens and secondly with Leishmania amazonensis antigens. in the first adsorption with H. capsulatum, reactivity to gp43 fell to 12.8% in the rural group and to 0% in the urban group. in the succeeding adsorption with L. amazonensis, this reactivity fell to 12.3% in the rural group. There was a statistically greater proportion of persons with gp43-reactive antibodies in rural group than in the urban group, indicating that rural residents had frequently become exposed to Pb and contracted primary, subclinical PCM. the present report is the first epidemiological study using ELISA to detect antibodies against gp43 in blood donors.en
dc.description.affiliationUniv Estadual Londrina, CCB, Dept Ciencias Patol, BR-86051970 Londrina, PR, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Coordinat Higher Level Profess Improvement Progra, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Immunogenet & Expt Transplantat Lab, São Paulo, Brazil
dc.description.affiliationHemoctr Reg Londrina, Londrina, PR, Brazil
dc.description.affiliationFdn Pro Sanguel, Hemoctr São Paulo, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Coordinat Higher Level Profess Improvement Progra, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent387-391
dc.identifierhttp://dx.doi.org/10.1080/mmy.40.4.387.391
dc.identifier.citationMedical Mycology. Oxford: B I O S Scientific Publishers Ltd, v. 40, n. 4, p. 387-391, 2002.
dc.identifier.doi10.1080/mmy.40.4.387.391
dc.identifier.issn1369-3786
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26944
dc.identifier.wosWOS:000177706100006
dc.language.isoeng
dc.publisherB I O S Scientific Publishers Ltd
dc.relation.ispartofMedical Mycology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectparacococcidioidomycosis-infectionen
dc.subjectantibodiesen
dc.subjectblood donorsen
dc.subjectParacoccidioides brasiliensisen
dc.subjectgp43en
dc.subjectexoantigensen
dc.subjectELISAen
dc.titleParacoccidioides brasiliensis-reactive antibodies in Brazilian blood donorsen
dc.typeinfo:eu-repo/semantics/article
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