Anti-PGL1 salivary IgA/IgM, serum IgG/IgM, and nasal Mycobacterium leprae DNA in individuals with household contact with leprosy

dc.contributor.authorCabral, Paula Brito e
dc.contributor.authorCunha Junior, Jose Evandro
dc.contributor.authorMacedo, Alexandre Casimiro de
dc.contributor.authorAlves, Alexandre Rodrigues
dc.contributor.authorGoncalves, Thially Braga
dc.contributor.authorCabral, Tereza Cristina Brito e
dc.contributor.authorGondim, Ana Paula Soares
dc.contributor.authorMoraes-Pinto, Maria Isabel de [UNIFESP]
dc.contributor.authorOseki, Karen Tubono [UNIFESP]
dc.contributor.authorCamara, Lilia Maria Carneiro
dc.contributor.authorRabenhorst, Silvia Helena Barem
dc.contributor.authorNagao-Dias, Aparecida Tiemi [UNIFESP]
dc.contributor.institutionUniv Fed Ceara
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:34:36Z
dc.date.available2016-01-24T14:34:36Z
dc.date.issued2013-11-01
dc.description.abstractObjectives: Leprosy household contacts represent a group at high risk of developing the disease. the aim of this study was to detect Mycobacterium leprae subclinical infection in this group through serological and molecular parameters.Methods: Serum anti-PGL1 IgG/IgM and salivary anti-PGL1 IgA/IgM was investigated using an ELISA, and nasal carriage of M. leprae DNA was detected by PCR, in leprosy household contacts of paucibacillary (PB) and multibacillary (MB) household leprosy patients (n = 135), their index cases (n = 30), and in persons living in a low endemic city (n = 17).Results: Salivary anti-PGL1 IgA and IgM and serum anti-PGL1 IgG showed good correlation comparing contacts and index cases (p < 0.01, p < 0.005, and p < 0.0001, respectively). This was not observed for serum anti-PGL1 IgM (p > 0.05). A high frequency of anti-PGL1 IgM positivity was found in IgG-negative samples (p < 0.0001). for IgG-positive samples, IgM antibodies were also positive in most of the samples. None of the 17 volunteers living in a low endemic city presented seropositivity for IgG; however, two of them showed positivity for anti-PGL1 IgM. M. leprae DNA was found in the nasal swabs of nine out of the 85 MB household leprosy contacts (10.6%) and in three out of the 50 PB household leprosy contacts (6.0%).Conclusion: We strongly suggest that serum IgG/IgM and salivary anti-PGL1 IgA/IgM measurements are used to follow leprosy household contacts. (C) 2013 International Society for Infectious Diseases. Published by Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Fed Ceara, Fac Pharm, Dept Clin Anal & Toxicol, BR-60430370 Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Ceara, Fac Pharm, Dept Pharm, BR-60430370 Fortaleza, Ceara, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Div Pediat Infect Dis, São Paulo, Brazil
dc.description.affiliationUniv Fed Ceara, Fac Med, Dept Pathol, BR-60430370 Fortaleza, Ceara, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Pediat Infect Dis, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDCNPq: 472.471/2007-4
dc.format.extentE1005-E1010
dc.identifierhttp://dx.doi.org/10.1016/j.ijid.2013.05.011
dc.identifier.citationInternational Journal of Infectious Diseases. Oxford: Elsevier B.V., v. 17, n. 11, p. E1005-E1010, 2013.
dc.identifier.doi10.1016/j.ijid.2013.05.011
dc.identifier.fileWOS000325986700014.pdf
dc.identifier.issn1201-9712
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36891
dc.identifier.wosWOS:000325986700014
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Journal of Infectious Diseases
dc.rightsAcesso aberto
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectMycobacterium leprae DNAen
dc.subjectSerum and salivary anti-PGL1en
dc.subjectLeprosy household contactsen
dc.titleAnti-PGL1 salivary IgA/IgM, serum IgG/IgM, and nasal Mycobacterium leprae DNA in individuals with household contact with leprosyen
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